Miscanthus sinensis germination images

Images of germination in Miscanthus sinensis seed, under a range of treatments.
A set of ~5,000 Miscanthus sinensis seed germinating over 11 days.

Photographed using a (Nikon D90) DSLR at a resolution of 4,288 x 2,848 pixels.

Each seed image is ~282 x 341 pixels.

The seed were sterilised with a low concentration bleach solution (0.5% Sodium Hypochlorite).

The seed are from an experiment which treated them with standard plant hormones (gibberellic acid (from 0.15 to 750 ppm), 1-naphthaleneacetic acid (from 0.01 to 200 ppm), epibrassinolide (from 0.001 to 2 ppm1) and abscisic acid (from 0.05 to 60 ppm)), or had induced water stresses (NaCl and polyethylene glycol (8000 and 4000 ppm respectively) producing water potentials of up to -4.1 MPa) or they were stratified.

These treatments produce a diverse and challenging range of germination phenotypes with which to test image analysis.

All images were scored by one person for consistency and the human score of this image set was the only reference point to which the computer score was compared.

The time of germination and mold were scored with 9999 used for seed that did not germinate or go moldy.

Stem and root measurements were also added on the 11th day.