A comparison of two approaches to extracting Cryptosporidium DNA from human stools as measured by a real-time PCR assay

K. Elwin; G. Robinson; S. J. Hadfield; H. V. Fairclough; M. Iturriza-Gomara; R. M. Chalmers

doi:10.1016/j.mimet.2012.02.006

A comparison of two approaches to extracting Cryptosporidium DNA from human stools as measured by a real-time PCR assay

K. Elwin, G. Robinson, S. J. Hadfield, H. V. Fairclough, M. Iturriza-Gomara, R. M. Chalmers

Gwyddorau Bywyd

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

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Direct extraction of Cryptosporidium DNA from 46 stools by bead-beating, guanidine thiocyanate and silica purification provided slightly lower PCR positivity (93.5% vs. 100%) and higher threshold cycle values (mean 34.93 vs. 28.03; P = 0.00) than spin-column extraction from boiled, semi-purified oocyst suspensions. However, direct extraction is cheaper, and amenable to automation.

Iaith wreiddiol	Saesneg
Tudalennau (o-i)	38-40
Nifer y tudalennau	3
Cyfnodolyn	Journal of Microbiological Methods
Cyfrol	89
Rhif cyhoeddi	1
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1016/j.mimet.2012.02.006
Statws	Cyhoeddwyd - 01 Ebr 2012

Mynediad at Ddogfen

10.1016/j.mimet.2012.02.006

Cysylltiad parhaol

Dolen barhaus

Ffeiliau eraill a chysylltiadau

http://hdl.handle.net/2160/8117

Dyfynnu hyn

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abstract = "Direct extraction of Cryptosporidium DNA from 46 stools by bead-beating, guanidine thiocyanate and silica purification provided slightly lower PCR positivity (93.5% vs. 100%) and higher threshold cycle values (mean 34.93 vs. 28.03; P = 0.00) than spin-column extraction from boiled, semi-purified oocyst suspensions. However, direct extraction is cheaper, and amenable to automation.",

keywords = "Cryptosporidium, DNA extraction, Real time PCR",

author = "K. Elwin and G. Robinson and Hadfield, {S. J.} and Fairclough, {H. V.} and M. Iturriza-Gomara and Chalmers, {R. M.}",

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AU - Elwin, K.

AU - Robinson, G.

AU - Hadfield, S. J.

AU - Fairclough, H. V.

AU - Iturriza-Gomara, M.

AU - Chalmers, R. M.

PY - 2012/4/1

Y1 - 2012/4/1

N2 - Direct extraction of Cryptosporidium DNA from 46 stools by bead-beating, guanidine thiocyanate and silica purification provided slightly lower PCR positivity (93.5% vs. 100%) and higher threshold cycle values (mean 34.93 vs. 28.03; P = 0.00) than spin-column extraction from boiled, semi-purified oocyst suspensions. However, direct extraction is cheaper, and amenable to automation.

AB - Direct extraction of Cryptosporidium DNA from 46 stools by bead-beating, guanidine thiocyanate and silica purification provided slightly lower PCR positivity (93.5% vs. 100%) and higher threshold cycle values (mean 34.93 vs. 28.03; P = 0.00) than spin-column extraction from boiled, semi-purified oocyst suspensions. However, direct extraction is cheaper, and amenable to automation.

KW - Cryptosporidium

KW - DNA extraction

KW - Real time PCR

UR - http://hdl.handle.net/2160/8117

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DO - 10.1016/j.mimet.2012.02.006

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A comparison of two approaches to extracting Cryptosporidium DNA from human stools as measured by a real-time PCR assay

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Ffeiliau eraill a chysylltiadau

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