A DNA Barcode for land plants

Peter M Hollingsworth , Laura L. Forrest, John L. Spouge, Mehrdad Hajibabaei, Sujeevan Ratnasingham, Michelle van der Bank, Mark W. Chase, Robyn S. Cowan, David L. Erickson, Aron J. Fazekas, Sean W. Graham, Karen E. James, Ki-Joong Kim, W. John Kress, Harald Schneider, Jonathan van AlphenStahl, Spencer C. H. Barrett, Cassio van den Berg, Diego Bogarin, Kevin S. BurgessKenneth M. Cameron, Mark Carine, Juliana Chacon, Alexandra Clark, James J. Clarkson, Ferozah Conrad, Dion S. Devey, Terry A. J. Hedderson, C. S. Ford, Michelle L. Hollingsworth, Brian C. Husband, Laura J. Kelly, Prasad R. Kesanakurti, Jung Sung Kim, Young-Dong Kim, Renaud Lahaye, Hae-Lim Lee, David G. Long, Santiago Madriñán, Olivier Maurin, Isabelle Meusnier, Steven G. Newmaster, Chong-Wook Park, Diana M. Percy, Gitte Petersen, J. E. Richardson, Gerardo A. Salazar, Vincent Savolainen, Ole Seberg, M. J. Wilkinson, Dong-Keun Yi, Damon P. Little

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

2136 Dyfyniadau(SciVal)

Crynodeb

DNA barcoding involves sequencing a standard region of DNA as a tool for species identification. However, there has been no agreement on which region(s) should be used for barcoding land plants. To provide a community recommendation on a standard plant barcode, we have compared the performance of 7 leading candidate plastid DNA regions (atpF–atpH spacer, matK gene, rbcL gene, rpoB gene, rpoC1 gene, psbK–psbI spacer, and trnH–psbA spacer). Based on assessments of recoverability, sequence quality, and levels of species discrimination, we recommend the 2-locus combination of rbcL+matK as the plant barcode. This core 2-locus barcode will provide a universal framework for the routine use of DNA sequence data to identify specimens and contribute toward the discovery of overlooked species of land plants.
Iaith wreiddiolSaesneg
Tudalennau (o-i)12794-12797
Nifer y tudalennau4
CyfnodolynProceedings of the National Academy of Sciences of the United States of America
Cyfrol106
Rhif cyhoeddi31
Dyddiad ar-lein cynnar30 Gorff 2009
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 2009

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