TY - JOUR
T1 - A genetic map of the apospory-region in Brachiaria hybrids
T2 - identification of two markers closely associated with the trait
AU - Pessino, Silvina C.
AU - Evans, C.
AU - Ortiz, Juan Pablo A.
AU - Armstead, Ian
AU - do Valle, Cacilda B.
AU - Hayward, Michael D.
PY - 1998
Y1 - 1998
N2 - The objective of this work was to identify molecular markers tightly linked to the gene controlling apospory in a hybrid population derived from a cross between apomictic Brachiaria brizantha and sexual Brachiaria ruziziensis. Since a maize chromosome 5 linkage group had previously been associated with the apomixis locus in Brachiaria we used twenty-five RFLP clones that map in detail maize chromosome 5 to cover systematically all syntenic regions of the hybrid genome. Forty-six AFLP markers were also generated. Three RFLP markers (detected by rz567, rz273, and cdo507 and two AFLP markers (PAM52-5 and PAM49-13) appeared to be related to the apo-region. Segregation data, together with previously reported data (corresponding to RFLP markers umc147, umc72, csu134, csu149 and RAPD marker OPC4) were used to generate a complete map of the region. Markers PAM52-5 and PAM49-13 were located respectively at 1.2 cM and 5.7 cM either side of the target locus. The map shows close synteny to regions of maize chromosome 5 and rice chromosome 2.
AB - The objective of this work was to identify molecular markers tightly linked to the gene controlling apospory in a hybrid population derived from a cross between apomictic Brachiaria brizantha and sexual Brachiaria ruziziensis. Since a maize chromosome 5 linkage group had previously been associated with the apomixis locus in Brachiaria we used twenty-five RFLP clones that map in detail maize chromosome 5 to cover systematically all syntenic regions of the hybrid genome. Forty-six AFLP markers were also generated. Three RFLP markers (detected by rz567, rz273, and cdo507 and two AFLP markers (PAM52-5 and PAM49-13) appeared to be related to the apo-region. Segregation data, together with previously reported data (corresponding to RFLP markers umc147, umc72, csu134, csu149 and RAPD marker OPC4) were used to generate a complete map of the region. Markers PAM52-5 and PAM49-13 were located respectively at 1.2 cM and 5.7 cM either side of the target locus. The map shows close synteny to regions of maize chromosome 5 and rice chromosome 2.
UR - http://www.scopus.com/inward/record.url?scp=0031835342&partnerID=8YFLogxK
U2 - 10.1111/j.1601-5223.1998.00153.x
DO - 10.1111/j.1601-5223.1998.00153.x
M3 - Article
SN - 0018-0661
VL - 128
SP - 153
EP - 158
JO - Hereditas
JF - Hereditas
IS - 2
ER -