A multiplex polymerase chain reaction for distinguishing Mycobacterium tuberculosis from Mycobacterium tuberculosis complex

Kathryn Sinclair; Janet A. Challans; Rudovick R. Kazwala; R. Glyn Hewinson; J. Michael Sharp

doi:10.1016/S0890-8508(95)91524-9

A multiplex polymerase chain reaction for distinguishing Mycobacterium tuberculosis from Mycobacterium tuberculosis complex

Kathryn Sinclair^*, Janet A. Challans, Rudovick R. Kazwala, R. Glyn Hewinson, J. Michael Sharp

^*Awdur cyfatebol y gwaith hwn

TB buchol, clefydau a rheolaeth

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

12 Dyfyniadau (Scopus)

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A multiplex polymerase chain reaction has been developed which is able to distinguish Mycobacterium tuberculosis from other members of the M. tuberculosis complex. The assay is based on the simultaneous amplification of two different targets: a 396bp region from the mtp40 species-specific gene sequence of M. tuberculosis and a 245bp fragment from the M. tuberculosis complex insertion sequence IS986. Results have been obtained for 54 mycobacterial strains including five non-M. tuberculosis complex isolates. All 49 strains of the M. tuberculosis complex were positive for IS986 but only the 27 M. tuberculosis isolates were positive for both IS986 and mtp40.

Iaith wreiddiol	Saesneg
Tudalennau (o-i)	291-295
Nifer y tudalennau	5
Cyfnodolyn	Molecular and Cellular Probes
Cyfrol	9
Rhif cyhoeddi	5
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1016/S0890-8508(95)91524-9
Statws	Cyhoeddwyd - 01 Hyd 1995

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10.1016/S0890-8508(95)91524-9

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Link to publication in Scopus

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@article{eda39e3d6afe46dc8a44e9fbb6c2aeb2,

title = "A multiplex polymerase chain reaction for distinguishing Mycobacterium tuberculosis from Mycobacterium tuberculosis complex",

abstract = "A multiplex polymerase chain reaction has been developed which is able to distinguish Mycobacterium tuberculosis from other members of the M. tuberculosis complex. The assay is based on the simultaneous amplification of two different targets: a 396bp region from the mtp40 species-specific gene sequence of M. tuberculosis and a 245bp fragment from the M. tuberculosis complex insertion sequence IS986. Results have been obtained for 54 mycobacterial strains including five non-M. tuberculosis complex isolates. All 49 strains of the M. tuberculosis complex were positive for IS986 but only the 27 M. tuberculosis isolates were positive for both IS986 and mtp40.",

keywords = "Diagnosis, M. tuberculosis complex, Polymerase chain reaction, Tuberculosis/diagnosis, Diagnosis, Differential, Base Sequence, Humans, Polymerase Chain Reaction, Molecular Sequence Data, Mycobacterium/isolation & purification, Mycobacterium tuberculosis/isolation & purification, DNA Primers",

author = "Kathryn Sinclair and Challans, {Janet A.} and Kazwala, {Rudovick R.} and Hewinson, {R. Glyn} and Sharp, {J. Michael}",

year = "1995",

month = oct,

day = "1",

doi = "10.1016/S0890-8508(95)91524-9",

language = "English",

volume = "9",

pages = "291--295",

journal = "Molecular and Cellular Probes",

issn = "0890-8508",

publisher = "Elsevier",

number = "5",

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TY - JOUR

T1 - A multiplex polymerase chain reaction for distinguishing Mycobacterium tuberculosis from Mycobacterium tuberculosis complex

AU - Sinclair, Kathryn

AU - Challans, Janet A.

AU - Kazwala, Rudovick R.

AU - Hewinson, R. Glyn

AU - Sharp, J. Michael

PY - 1995/10/1

Y1 - 1995/10/1

N2 - A multiplex polymerase chain reaction has been developed which is able to distinguish Mycobacterium tuberculosis from other members of the M. tuberculosis complex. The assay is based on the simultaneous amplification of two different targets: a 396bp region from the mtp40 species-specific gene sequence of M. tuberculosis and a 245bp fragment from the M. tuberculosis complex insertion sequence IS986. Results have been obtained for 54 mycobacterial strains including five non-M. tuberculosis complex isolates. All 49 strains of the M. tuberculosis complex were positive for IS986 but only the 27 M. tuberculosis isolates were positive for both IS986 and mtp40.

AB - A multiplex polymerase chain reaction has been developed which is able to distinguish Mycobacterium tuberculosis from other members of the M. tuberculosis complex. The assay is based on the simultaneous amplification of two different targets: a 396bp region from the mtp40 species-specific gene sequence of M. tuberculosis and a 245bp fragment from the M. tuberculosis complex insertion sequence IS986. Results have been obtained for 54 mycobacterial strains including five non-M. tuberculosis complex isolates. All 49 strains of the M. tuberculosis complex were positive for IS986 but only the 27 M. tuberculosis isolates were positive for both IS986 and mtp40.

KW - Diagnosis

KW - M. tuberculosis complex

KW - Polymerase chain reaction

KW - Tuberculosis/diagnosis

KW - Diagnosis, Differential

KW - Base Sequence

KW - Humans

KW - Polymerase Chain Reaction

KW - Molecular Sequence Data

KW - Mycobacterium/isolation & purification

KW - Mycobacterium tuberculosis/isolation & purification

KW - DNA Primers

UR - http://www.scopus.com/inward/record.url?scp=0028818345&partnerID=8YFLogxK

U2 - 10.1016/S0890-8508(95)91524-9

DO - 10.1016/S0890-8508(95)91524-9

M3 - Article

C2 - 8569767

AN - SCOPUS:0028818345

SN - 0890-8508

VL - 9

SP - 291

EP - 295

JO - Molecular and Cellular Probes

JF - Molecular and Cellular Probes

IS - 5

ER -

A multiplex polymerase chain reaction for distinguishing Mycobacterium tuberculosis from Mycobacterium tuberculosis complex

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Mynediad at Ddogfen

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Ffeiliau eraill a chysylltiadau

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