A single dose of killed Mycobacterium bovis BCG in a novel class of adjuvant (Novasome™) protects guinea pigs from lethal tuberculosis

Mark A. Chambers; D. Craig Wright; Joan Brisker; Ann Williams; Graham Hatch; Dolores Gavier-Widén; Graham Hall; Philip D. Marsh; R. Glyn Hewinson

doi:10.1016/j.vaccine.2003.05.002

A single dose of killed Mycobacterium bovis BCG in a novel class of adjuvant (Novasome™) protects guinea pigs from lethal tuberculosis

Mark A. Chambers^*, D. Craig Wright, Joan Brisker, Ann Williams, Graham Hatch, Dolores Gavier-Widén, Graham Hall, Philip D. Marsh, R. Glyn Hewinson

^*Awdur cyfatebol y gwaith hwn

TB buchol, clefydau a rheolaeth

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

Crynodeb

The only vaccine currently available for the prevention of tuberculosis in man is a live attenuated vaccine, bacille Calmette-Guerin (BCG), derived from Mycobacterium bovis. Concerns over the lack of the universal efficacy and safety of BCG have resulted in efforts to develop a new generation of TB vaccines. Historically, killed whole-cell preparations of mycobacteria have been ineffective vaccines. We revisited the potential of killed whole-cell vaccines by comparing their efficacy with live BCG Pasteur in a guinea pig challenge model. BCG Pasteur was inactivated with a low concentration of formalin and showed to be non-viable in culture or severe combined immunodeficient mice. Formalin-inactivated BCG was mixed with non-phospholipid liposome adjuvants (Novasomes™) and administered to guinea pigs as a single subcutaneous inoculation. All formulations were well tolerated and one conferred a significant survival advantage against lethal aerogenic challenge with M. bovis. Crown

Iaith wreiddiol	Saesneg
Tudalennau (o-i)	1063-1071
Nifer y tudalennau	9
Cyfnodolyn	Vaccine
Cyfrol	22
Rhif cyhoeddi	8
Dyddiad ar-lein cynnar	14 Hyd 2003
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1016/j.vaccine.2003.05.002
Statws	Cyhoeddwyd - 25 Chwef 2004

NDC y CU

Mae’r allbwn hwn yn cyfrannu at y Nod(au) Datblygu Cynaliadwy canlynol

Mynediad at Ddogfen

10.1016/j.vaccine.2003.05.002

Cysylltiad parhaol

Dolen barhaus

Ffeiliau eraill a chysylltiadau

Link to publication in Scopus

Dyfynnu hyn

@article{fa4744d0a8f141ae99f135eaad47c01c,

title = "A single dose of killed Mycobacterium bovis BCG in a novel class of adjuvant (Novasome{\texttrademark}) protects guinea pigs from lethal tuberculosis",

abstract = "The only vaccine currently available for the prevention of tuberculosis in man is a live attenuated vaccine, bacille Calmette-Guerin (BCG), derived from Mycobacterium bovis. Concerns over the lack of the universal efficacy and safety of BCG have resulted in efforts to develop a new generation of TB vaccines. Historically, killed whole-cell preparations of mycobacteria have been ineffective vaccines. We revisited the potential of killed whole-cell vaccines by comparing their efficacy with live BCG Pasteur in a guinea pig challenge model. BCG Pasteur was inactivated with a low concentration of formalin and showed to be non-viable in culture or severe combined immunodeficient mice. Formalin-inactivated BCG was mixed with non-phospholipid liposome adjuvants (Novasomes{\texttrademark}) and administered to guinea pigs as a single subcutaneous inoculation. All formulations were well tolerated and one conferred a significant survival advantage against lethal aerogenic challenge with M. bovis. Crown",

keywords = "Liposome, M. bovis, Vaccine",

author = "Chambers, {Mark A.} and Wright, {D. Craig} and Joan Brisker and Ann Williams and Graham Hatch and Dolores Gavier-Wid{\'e}n and Graham Hall and Marsh, {Philip D.} and Hewinson, {R. Glyn}",

year = "2004",

month = feb,

day = "25",

doi = "10.1016/j.vaccine.2003.05.002",

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journal = "Vaccine",

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T1 - A single dose of killed Mycobacterium bovis BCG in a novel class of adjuvant (Novasome™) protects guinea pigs from lethal tuberculosis

AU - Chambers, Mark A.

AU - Wright, D. Craig

AU - Brisker, Joan

AU - Williams, Ann

AU - Hatch, Graham

AU - Gavier-Widén, Dolores

AU - Hall, Graham

AU - Marsh, Philip D.

AU - Hewinson, R. Glyn

PY - 2004/2/25

Y1 - 2004/2/25

N2 - The only vaccine currently available for the prevention of tuberculosis in man is a live attenuated vaccine, bacille Calmette-Guerin (BCG), derived from Mycobacterium bovis. Concerns over the lack of the universal efficacy and safety of BCG have resulted in efforts to develop a new generation of TB vaccines. Historically, killed whole-cell preparations of mycobacteria have been ineffective vaccines. We revisited the potential of killed whole-cell vaccines by comparing their efficacy with live BCG Pasteur in a guinea pig challenge model. BCG Pasteur was inactivated with a low concentration of formalin and showed to be non-viable in culture or severe combined immunodeficient mice. Formalin-inactivated BCG was mixed with non-phospholipid liposome adjuvants (Novasomes™) and administered to guinea pigs as a single subcutaneous inoculation. All formulations were well tolerated and one conferred a significant survival advantage against lethal aerogenic challenge with M. bovis. Crown

AB - The only vaccine currently available for the prevention of tuberculosis in man is a live attenuated vaccine, bacille Calmette-Guerin (BCG), derived from Mycobacterium bovis. Concerns over the lack of the universal efficacy and safety of BCG have resulted in efforts to develop a new generation of TB vaccines. Historically, killed whole-cell preparations of mycobacteria have been ineffective vaccines. We revisited the potential of killed whole-cell vaccines by comparing their efficacy with live BCG Pasteur in a guinea pig challenge model. BCG Pasteur was inactivated with a low concentration of formalin and showed to be non-viable in culture or severe combined immunodeficient mice. Formalin-inactivated BCG was mixed with non-phospholipid liposome adjuvants (Novasomes™) and administered to guinea pigs as a single subcutaneous inoculation. All formulations were well tolerated and one conferred a significant survival advantage against lethal aerogenic challenge with M. bovis. Crown

KW - Liposome

KW - M. bovis

KW - Vaccine

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U2 - 10.1016/j.vaccine.2003.05.002

DO - 10.1016/j.vaccine.2003.05.002

M3 - Article

C2 - 15161084

AN - SCOPUS:1242270634

SN - 0264-410X

VL - 22

SP - 1063

EP - 1071

JO - Vaccine

JF - Vaccine

IS - 8

ER -

A single dose of killed Mycobacterium bovis BCG in a novel class of adjuvant (Novasome™) protects guinea pigs from lethal tuberculosis

Crynodeb

NDC y CU

Mynediad at Ddogfen

Cysylltiad parhaol

Ffeiliau eraill a chysylltiadau

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Dyfynnu hyn