@article{fe0de01561154c86b14424d9b3040a9d,
title = "A xylanase produced by the rumen anaerobic protozoan Polyplastron multivesiculatum shows close sequence similarity to family 11 xylanases from Gram-positive bacteria",
abstract = "We report for the first time the cloning and characterisation of a protozoal enzyme involved in plant cell wall polysaccharide degradation. A cDNA library was constructed from the ruminal protozoan Polyplastron multivesiculatum and a stable clone expressing xylanase activity was isolated. The encoded enzyme belongs to the glycoside hydrolase family 11, and phylogenetic analysis indicates a closer relationship with catalytic domains from Gram-positive bacteria than the other fibrolytic eukaryotes from the rumen, the anaerobic fungi.",
keywords = "POPULATIONS, PROTEIN, rumen, CELLULOLYTIC BACTERIA, protozoa, CELLULASES, GENE, FUNGI, DIGESTION, ENZYMES, Polyplastron multivesiculatum, DOMAINS, SHEEP, xylanase, Protozoa, Rumen, Xylanase",
author = "Estelle Devillard and Newbold, \{C. J.\} and Scott, \{Karen P.\} and Evelyne Forano and Wallace, \{R. John\} and Jean-Pierre Jouany and Flint, \{Harry J.\}",
note = "Funding Information: We are grateful for joint support from INRA (France) and from the Scottish Office Agriculture, Fisheries and Environment Department (SOAFED, UK) for this project. E.D. is supported by a grant {\textquoteleft}Cotutelle de th{\`e}se{\textquoteright} from the French MENRT. We thank L. Millet for the gift of fungal DNA.",
year = "1999",
month = dec,
day = "1",
doi = "10.1016/S0378-1097(99)00525-X",
language = "English",
volume = "181",
pages = "145--152",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Oxford University Press",
number = "1",
}