TY - JOUR
T1 - African 2, a clonal complex of Mycobacterium bovis epidemiologically important in East Africa
AU - Berg, Stefan
AU - Garcia-Pelayo, M. Carmen
AU - Müller, Borna
AU - Hailu, Elena
AU - Asiimwe, Benon
AU - Kremer, Kristin
AU - Dale, James
AU - Boniotti, M. Beatrice
AU - Rodriguez, Sabrina
AU - Hilty, Markus
AU - Rigouts, Leen
AU - Firdessa, Rebuma
AU - MacHado, Adelina
AU - Mucavele, Custodia
AU - Ngandolo, Bongo Nare Richard
AU - Bruchfeld, Judith
AU - Boschiroli, Laura
AU - Müller, Annélle
AU - Sahraoui, Naima
AU - Pacciarini, Maria
AU - Cadmus, Simeon
AU - Joloba, Moses
AU - Van Soolingen, Dick
AU - Michel, Anita L.
AU - Djønne, Berit
AU - Aranaz, Alicia
AU - Zinsstag, Jakob
AU - Van Helden, Paul
AU - Portaels, Françoise
AU - Kazwala, Rudovick
AU - Källenius, Gunilla
AU - Hewinson, R. Glyn
AU - Aseffa, Abraham
AU - Gordon, Stephen V.
AU - Smith, Noel H.
PY - 2011/2/1
Y1 - 2011/2/1
N2 - We have identified a clonal complex of Mycobacterium bovis isolated at high frequency from cattle in Uganda, Burundi, Tanzania, and Ethiopia. We have named this related group of M. bovis strains the African 2 (Af2) clonal complex of M. bovis. Af2 strains are defined by a specific chromosomal deletion (RDAf2) and can be identified by the absence of spacers 3 to 7 in their spoligotype patterns. Deletion analysis of M. bovis isolates from Algeria, Mali, Chad, Nigeria, Cameroon, South Africa, and Mozambique did not identify any strains of the Af2 clonal complex, suggesting that this clonal complex of M. bovis is localized in East Africa. The specific spoligotype pattern of the Af2 clonal complex was rarely identified among isolates from outside Africa, and the few isolates that were found and tested were intact at the RDAf2 locus. We conclude that the Af2 clonal complex is localized to cattle in East Africa. We found that strains of the Af2 clonal complex of M. bovis have, in general, four or more copies of the insertion sequence IS6110, in contrast to the majority of M. bovis strains isolated from cattle, which are thought to carry only one or a few copies.
AB - We have identified a clonal complex of Mycobacterium bovis isolated at high frequency from cattle in Uganda, Burundi, Tanzania, and Ethiopia. We have named this related group of M. bovis strains the African 2 (Af2) clonal complex of M. bovis. Af2 strains are defined by a specific chromosomal deletion (RDAf2) and can be identified by the absence of spacers 3 to 7 in their spoligotype patterns. Deletion analysis of M. bovis isolates from Algeria, Mali, Chad, Nigeria, Cameroon, South Africa, and Mozambique did not identify any strains of the Af2 clonal complex, suggesting that this clonal complex of M. bovis is localized in East Africa. The specific spoligotype pattern of the Af2 clonal complex was rarely identified among isolates from outside Africa, and the few isolates that were found and tested were intact at the RDAf2 locus. We conclude that the Af2 clonal complex is localized to cattle in East Africa. We found that strains of the Af2 clonal complex of M. bovis have, in general, four or more copies of the insertion sequence IS6110, in contrast to the majority of M. bovis strains isolated from cattle, which are thought to carry only one or a few copies.
UR - https://www.scopus.com/pages/publications/78751480655
U2 - 10.1128/JB.00750-10
DO - 10.1128/JB.00750-10
M3 - Article
C2 - 21097608
AN - SCOPUS:78751480655
SN - 0021-9193
VL - 193
SP - 670
EP - 678
JO - Journal of Bacteriology
JF - Journal of Bacteriology
IS - 3
ER -