An improved fluorescent amplified fragment length polymorphism method for typing Mycobacterium tuberculosis

Michael Shemko; Zack Fang; Graham MacIntire; Royston Goodacre; Nandini Shetty; Vanya Gant; Yankuba Kassama

doi:10.1128/JCM.44.1.288-289.2006

An improved fluorescent amplified fragment length polymorphism method for typing Mycobacterium tuberculosis

Michael Shemko, Zack Fang, Graham MacIntire, Royston Goodacre, Nandini Shetty, Vanya Gant, Yankuba Kassama

Gwyddorau Bywyd

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

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We have evaluated fluorescent amplified fragment length polymorphism (FAFLP) fingerprinting as a complementary technique for genotyping Mycobacterium tuberculosis, which may aid in the elucidation of the transmission dynamics of tuberculosis. Earlier FAFLP studies (1, 2, 3, 5) broadly employed EcoRI and MseI restriction enzymes, which are known to have a low cleavage frequency for GC genomes of >65 mol% (4). By contrast, BamHI and MspI restriction endonucleases were used in this study because they have a higher cleavage frequency (as judged by in silico calculations) for the M. tuberculosis genome and do not show polymorphisms within IS6110/IS986

Iaith wreiddiol	Saesneg
Tudalennau (o-i)	288-289
Nifer y tudalennau	2
Cyfnodolyn	Journal of Clinical Microbiology
Cyfrol	44
Rhif cyhoeddi	1
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1128/JCM.44.1.288-289.2006
Statws	Cyhoeddwyd - Ion 2006

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10.1128/JCM.44.1.288-289.2006

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@article{06311a5a9ba34e9b964883c67d0f18b6,

title = "An improved fluorescent amplified fragment length polymorphism method for typing Mycobacterium tuberculosis",

abstract = "We have evaluated fluorescent amplified fragment length polymorphism (FAFLP) fingerprinting as a complementary technique for genotyping Mycobacterium tuberculosis, which may aid in the elucidation of the transmission dynamics of tuberculosis. Earlier FAFLP studies (1, 2, 3, 5) broadly employed EcoRI and MseI restriction enzymes, which are known to have a low cleavage frequency for GC genomes of >65 mol% (4). By contrast, BamHI and MspI restriction endonucleases were used in this study because they have a higher cleavage frequency (as judged by in silico calculations) for the M. tuberculosis genome and do not show polymorphisms within IS6110/IS986",

author = "Michael Shemko and Zack Fang and Graham MacIntire and Royston Goodacre and Nandini Shetty and Vanya Gant and Yankuba Kassama",

note = "Kassama, Y., Shemko, M., Shetty, N., Fang, Z., MacIntire, G., Gant, V., Zumla, A., Goodacre, R. (2006). An improved fluorescent amplified fragment length polymorphism method for typing Mycobacterium tuberculosis. Journal of Clinical Microbiology, 44, (1), 288-289. Sponsorship: BBSRC",

year = "2006",

month = jan,

doi = "10.1128/JCM.44.1.288-289.2006",

language = "English",

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pages = "288--289",

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T1 - An improved fluorescent amplified fragment length polymorphism method for typing Mycobacterium tuberculosis

AU - Shemko, Michael

AU - Fang, Zack

AU - MacIntire, Graham

AU - Goodacre, Royston

AU - Shetty, Nandini

AU - Gant, Vanya

AU - Kassama, Yankuba

N1 - Kassama, Y., Shemko, M., Shetty, N., Fang, Z., MacIntire, G., Gant, V., Zumla, A., Goodacre, R. (2006). An improved fluorescent amplified fragment length polymorphism method for typing Mycobacterium tuberculosis. Journal of Clinical Microbiology, 44, (1), 288-289. Sponsorship: BBSRC

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N2 - We have evaluated fluorescent amplified fragment length polymorphism (FAFLP) fingerprinting as a complementary technique for genotyping Mycobacterium tuberculosis, which may aid in the elucidation of the transmission dynamics of tuberculosis. Earlier FAFLP studies (1, 2, 3, 5) broadly employed EcoRI and MseI restriction enzymes, which are known to have a low cleavage frequency for GC genomes of >65 mol% (4). By contrast, BamHI and MspI restriction endonucleases were used in this study because they have a higher cleavage frequency (as judged by in silico calculations) for the M. tuberculosis genome and do not show polymorphisms within IS6110/IS986

AB - We have evaluated fluorescent amplified fragment length polymorphism (FAFLP) fingerprinting as a complementary technique for genotyping Mycobacterium tuberculosis, which may aid in the elucidation of the transmission dynamics of tuberculosis. Earlier FAFLP studies (1, 2, 3, 5) broadly employed EcoRI and MseI restriction enzymes, which are known to have a low cleavage frequency for GC genomes of >65 mol% (4). By contrast, BamHI and MspI restriction endonucleases were used in this study because they have a higher cleavage frequency (as judged by in silico calculations) for the M. tuberculosis genome and do not show polymorphisms within IS6110/IS986

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DO - 10.1128/JCM.44.1.288-289.2006

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An improved fluorescent amplified fragment length polymorphism method for typing Mycobacterium tuberculosis

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