An NAD+-dependent glutamate dehydrogenase cloned from the ruminal ciliate protozoan, Entodinium caudatum

C. James Newbold; Neil R. McEwan; Roger E. Calza; Emilie N. Chareyron; Stéphane M. Duval; Sylvain C. P. Eschenlauer; Freda M. McIntosh; Nancy Nelson; Anthony J. Travis; R. John Wallace

doi:10.1016/j.femsle.2005.04.034

An NAD+-dependent glutamate dehydrogenase cloned from the ruminal ciliate protozoan, Entodinium caudatum

C. James Newbold, Neil R. McEwan, Roger E. Calza, Emilie N. Chareyron, Stéphane M. Duval, Sylvain C. P. Eschenlauer, Freda M. McIntosh, Nancy Nelson, Anthony J. Travis, R. John Wallace

Gwyddorau Bywyd

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

Crynodeb

An NAD+-dependent glutamate dehydrogenase (GDH; EC 1.4.1.24) was cloned from the ruminal ciliate protozoan, Entodinium caudatum. The gene had high sequence similarity to GDH genes from the Bacteroides (class) – a class of bacteria which is highly represented in the rumen. When expressed in Escherichia coli the enzyme had a high affinity for ammonia and α-ketoglutarate (apparent Km of 2.33 and 0.71 mM, respectively) and a low affinity for glutamate (apparent Km of 98 mM). GDH activity and GDH mRNA concentration were increased by incubating washed E. caudatum cells with ammonia and antibiotics. These results suggest that the GDH is an anabolic enzyme catalysing the assimilation of ammonia by E. caudatum in the rumen and that the gene was probably acquired by lateral gene transfer from a ruminal bacterium.

Iaith wreiddiol	Saesneg
Tudalennau (o-i)	113-121
Nifer y tudalennau	9
Cyfnodolyn	FEMS Microbiology Letters
Cyfrol	247
Rhif cyhoeddi	2
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1016/j.femsle.2005.04.034
Statws	Cyhoeddwyd - 01 Meh 2005

Mynediad at Ddogfen

10.1016/j.femsle.2005.04.034

http://hdl.handle.net/2160/3094

Cysylltiad parhaol

Dolen barhaus

Dyfynnu hyn

@article{f287612b839d479bb7c75f06f5416051,

title = "An NAD+-dependent glutamate dehydrogenase cloned from the ruminal ciliate protozoan, Entodinium caudatum",

abstract = "An NAD+-dependent glutamate dehydrogenase (GDH; EC 1.4.1.24) was cloned from the ruminal ciliate protozoan, Entodinium caudatum. The gene had high sequence similarity to GDH genes from the Bacteroides (class) – a class of bacteria which is highly represented in the rumen. When expressed in Escherichia coli the enzyme had a high affinity for ammonia and α-ketoglutarate (apparent Km of 2.33 and 0.71 mM, respectively) and a low affinity for glutamate (apparent Km of 98 mM). GDH activity and GDH mRNA concentration were increased by incubating washed E. caudatum cells with ammonia and antibiotics. These results suggest that the GDH is an anabolic enzyme catalysing the assimilation of ammonia by E. caudatum in the rumen and that the gene was probably acquired by lateral gene transfer from a ruminal bacterium.",

keywords = "Ammonia uptake, Entodinium caudatum, Glutamate dehydrogenase, Protozoa, Rumen",

author = "Newbold, {C. James} and McEwan, {Neil R.} and Calza, {Roger E.} and Chareyron, {Emilie N.} and Duval, {St{\'e}phane M.} and Eschenlauer, {Sylvain C. P.} and McIntosh, {Freda M.} and Nancy Nelson and Travis, {Anthony J.} and Wallace, {R. John}",

note = "Newbold, C. J., McEwan, N. R., Calza, R. E., Chareyron, E. N., Duval, S. M., Eschenlauer, S. C .P., McIntosh, F. M., Nelson, N., Travis, A. J., Wallace, R. J. (2005). An NAD+-dependent glutamate dehydrogenase cloned from the ruminal ciliate protozoan, Entodinium caudatum. FEMS Microbiology Letters, 247 (2), 113-121 Keywords: Ammonia uptake; Entodinium caudatum; Glutamate dehydrogenase; Protozoa; Rumen",

year = "2005",

month = jun,

day = "1",

doi = "10.1016/j.femsle.2005.04.034",

language = "English",

volume = "247",

pages = "113--121",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Wiley",

number = "2",

}

TY - JOUR

T1 - An NAD+-dependent glutamate dehydrogenase cloned from the ruminal ciliate protozoan, Entodinium caudatum

AU - Newbold, C. James

AU - McEwan, Neil R.

AU - Calza, Roger E.

AU - Chareyron, Emilie N.

AU - Duval, Stéphane M.

AU - Eschenlauer, Sylvain C. P.

AU - McIntosh, Freda M.

AU - Nelson, Nancy

AU - Travis, Anthony J.

AU - Wallace, R. John

N1 - Newbold, C. J., McEwan, N. R., Calza, R. E., Chareyron, E. N., Duval, S. M., Eschenlauer, S. C .P., McIntosh, F. M., Nelson, N., Travis, A. J., Wallace, R. J. (2005). An NAD+-dependent glutamate dehydrogenase cloned from the ruminal ciliate protozoan, Entodinium caudatum. FEMS Microbiology Letters, 247 (2), 113-121 Keywords: Ammonia uptake; Entodinium caudatum; Glutamate dehydrogenase; Protozoa; Rumen

PY - 2005/6/1

Y1 - 2005/6/1

N2 - An NAD+-dependent glutamate dehydrogenase (GDH; EC 1.4.1.24) was cloned from the ruminal ciliate protozoan, Entodinium caudatum. The gene had high sequence similarity to GDH genes from the Bacteroides (class) – a class of bacteria which is highly represented in the rumen. When expressed in Escherichia coli the enzyme had a high affinity for ammonia and α-ketoglutarate (apparent Km of 2.33 and 0.71 mM, respectively) and a low affinity for glutamate (apparent Km of 98 mM). GDH activity and GDH mRNA concentration were increased by incubating washed E. caudatum cells with ammonia and antibiotics. These results suggest that the GDH is an anabolic enzyme catalysing the assimilation of ammonia by E. caudatum in the rumen and that the gene was probably acquired by lateral gene transfer from a ruminal bacterium.

AB - An NAD+-dependent glutamate dehydrogenase (GDH; EC 1.4.1.24) was cloned from the ruminal ciliate protozoan, Entodinium caudatum. The gene had high sequence similarity to GDH genes from the Bacteroides (class) – a class of bacteria which is highly represented in the rumen. When expressed in Escherichia coli the enzyme had a high affinity for ammonia and α-ketoglutarate (apparent Km of 2.33 and 0.71 mM, respectively) and a low affinity for glutamate (apparent Km of 98 mM). GDH activity and GDH mRNA concentration were increased by incubating washed E. caudatum cells with ammonia and antibiotics. These results suggest that the GDH is an anabolic enzyme catalysing the assimilation of ammonia by E. caudatum in the rumen and that the gene was probably acquired by lateral gene transfer from a ruminal bacterium.

KW - Ammonia uptake

KW - Entodinium caudatum

KW - Glutamate dehydrogenase

KW - Protozoa

KW - Rumen

U2 - 10.1016/j.femsle.2005.04.034

DO - 10.1016/j.femsle.2005.04.034

M3 - Article

C2 - 15921862

SN - 0378-1097

VL - 247

SP - 113

EP - 121

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2

ER -

An NAD+-dependent glutamate dehydrogenase cloned from the ruminal ciliate protozoan, Entodinium caudatum

Crynodeb

Mynediad at Ddogfen

Cysylltiad parhaol

Ôl bys

Dyfynnu hyn