Prosiectau fesul blwyddyn
Crynodeb
Ethnopharmacology relevance
Maytenus ilicifolia is a Celastracea plant used in traditional medicine to alleviate digestive tract inflammatory disorders.
Aim of the study
We investigated anti-inflammatory properties of M. ilicifolia crude extract towards Caco-2 cell line, as a model of Toll-like Receptor 2 (TLR-2) inflammatory pathway.
Materials and Methods
Toxicity was assessed following culture of Caco-2 with M. ilicifolia, using apparent cell permeability and trans-epithelial electric resistance. Anti-inflammatory properties of M. ilicifolia were assessed through IL-8 secretion and TLR-2 associated gene expression of Caco-2 cells with or without an LTA challenge.
Results
M. ilicifolia was not toxic to Caco-2 cells. M. ilicifolia down-regulated TLR2 expression with and without LTA challenge but had no effect on other genes. Following LTA challenge of Caco-2 cells, 100 and 200 µg/mL M. ilicifolia abrogated IL-8 secretion.
Conclusions
We provide preliminary data for some M. ilicifolia anti-inflammatory properties. Further research must establish the full extent and mode of action on particular inflammatory pathways
Maytenus ilicifolia is a Celastracea plant used in traditional medicine to alleviate digestive tract inflammatory disorders.
Aim of the study
We investigated anti-inflammatory properties of M. ilicifolia crude extract towards Caco-2 cell line, as a model of Toll-like Receptor 2 (TLR-2) inflammatory pathway.
Materials and Methods
Toxicity was assessed following culture of Caco-2 with M. ilicifolia, using apparent cell permeability and trans-epithelial electric resistance. Anti-inflammatory properties of M. ilicifolia were assessed through IL-8 secretion and TLR-2 associated gene expression of Caco-2 cells with or without an LTA challenge.
Results
M. ilicifolia was not toxic to Caco-2 cells. M. ilicifolia down-regulated TLR2 expression with and without LTA challenge but had no effect on other genes. Following LTA challenge of Caco-2 cells, 100 and 200 µg/mL M. ilicifolia abrogated IL-8 secretion.
Conclusions
We provide preliminary data for some M. ilicifolia anti-inflammatory properties. Further research must establish the full extent and mode of action on particular inflammatory pathways
Iaith wreiddiol | Saesneg |
---|---|
Tudalennau (o-i) | 283-287 |
Nifer y tudalennau | 5 |
Cyfnodolyn | Journal of Ethnopharmacology |
Cyfrol | 209 |
Dyddiad ar-lein cynnar | 05 Awst 2017 |
Dynodwyr Gwrthrych Digidol (DOIs) | |
Statws | Cyhoeddwyd - 14 Medi 2017 |
Ôl bys
Gweld gwybodaeth am bynciau ymchwil 'Anti-inflammatory properties of an extract of M. ilicifolia in the human intestinal epithelial Caco-2 cell line'. Gyda’i gilydd, maen nhw’n ffurfio ôl bys unigryw.Proffiliau
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Ruth Wonfor
- Adran y Gwyddorau Bywyd - Lecturer in Animal and Equine Science
Unigolyn: Dysgu ac Ymchwil
Prosiectau
- 2 Wedi Gorffen
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BEACON Plus
Donnison, I. (Prif Ymchwilydd) & Shah, I. P. (Cyd-ymchwilydd)
01 Gorff 2015 → 31 Rhag 2019
Prosiect: Ymchwil a ariannwyd yn allanol
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Energy Grasses and Biorefining (EGB) ISPG
Donnison, I. (Prif Ymchwilydd) & Shah, I. P. (Cyd-ymchwilydd)
Biotechnology and Biological Sciences Research Council
01 Ebr 2012 → 31 Maw 2017
Prosiect: Ymchwil a ariannwyd yn allanol