Association and introgression mapping of quantitative traits in Lolium perenne: flowering time, forage quality and abiotic stress tolerance

L. Skot, D. Thorogood, I. P. Armstead, J. Humphreys, R. Sanderson, I. D. Thomas, J. A. Gallagher, L. B. Turner, M. W. Humphreys, M. O. Humphreys

Allbwn ymchwil: Cyfraniad at gynhadleddArall


Most important traits subject to genetic improvement in crop plants, including forage grasses, are governed by small effects of many genes. We are using two strategies, namely association and introgression mapping, for dissection and fine mapping of flowering time, forage quality and abiotic stress tolerance in the temperate forage grass Lolium perenne. Both complement conventional QTL mapping based on F2 or backcross mapping families. Association mapping uses populations of unknown pedigree to exploit the recombination events that have occurred over many generations, enabling a high mapping resolution. We have used a large number of genotypes to find associations between single nucleotide polymorphisms (SNPs) in two candidate genes for flowering time: the Hd1 (CONSTANS) and Hd3a (FT) rice orthologues in L. perenne. After accounting for population structure we identified highly significant statistical associations in both candidate genes with flowering time. We have validated these associations by analysing progeny from test crosses involving genotypes from the experimental population with genotypes of turf grass varieties, and by genotyping unrelated natural populations known to contrast in flowering time. Forage quality is another target in our association mapping program.
Introgression mapping exploits the ability of Lolium and Festuca to hybridise. Lolium plant lines have been established containing segments of Festuca chromosome 3 introgressed, since there is evidence to suggest that this chromosome in Festuca can provide allelic variants conferring improved drought tolerance to Lolium. We are exploiting the synteny with Chromosome 1 in rice to find likely candidate genes for further analysis and marker development.
Iaith wreiddiolSaesneg
StatwsCyhoeddwyd - 2008
DigwyddiadPlant and Animal Genomics Conference XVI - San Diego, Unol Daleithiau America
Hyd: 12 Ion 200816 Ion 2008


CynhadleddPlant and Animal Genomics Conference XVI
Gwlad/TiriogaethUnol Daleithiau America
DinasSan Diego
Cyfnod12 Ion 200816 Ion 2008

Ôl bys

Gweld gwybodaeth am bynciau ymchwil 'Association and introgression mapping of quantitative traits in Lolium perenne: flowering time, forage quality and abiotic stress tolerance'. Gyda’i gilydd, maen nhw’n ffurfio ôl bys unigryw.

Dyfynnu hyn