TY - JOUR
T1 - Association of immune responses of Zebu and Holstein-Friesian cattle and resistance to mycobacteria in a BCG challenge model
AU - Alcaraz-López, Omar Antonio
AU - Flores-Villalva, Susana
AU - Cortéz-Hernández, Omar
AU - Vigueras-Meneses, Guadalupe
AU - Carrisoza-Urbina, Jacobo
AU - Benítez-Guzmán, Alejandro
AU - Esquivel-Solís, Hugo
AU - Werling, Dirk
AU - Salguero Bodes, Francisco J.
AU - Vordemeier, Martin
AU - Villarreal-Ramos, Bernardo
AU - Gutiérrez-Pabello, José A.
N1 - Funding Information:
The authors wish to dedicate this manuscript to the memory of our friend and colleague Omar Antonio Alcaraz‐López, whose life has been sadly claimed by the currently ongoing COVID‐19 pandemic. This work was funded by the Consejo Nacional de Ciencia y Tecnología, México, through an Institutional Links grant: FONCICYT 277863; and the Birtish Council, through Institutional Links Newton Grant 276305277. The authors wish to thank colleagues at INIFAP, Queretaro for the excellent care and upkeep of the animals used in this experiment and all their help with the collection and processing of samples. The authors also wish to thank Mr Alejandro Nava and Ms Itzel Jiménez for their help in the handling of the animals at the time of inoculation. BVR and MV are Sêr Cymru II Professors at IBERS, AU, funded by the European Research Development Fund and Welsh Government.
Funding Information:
The authors wish to dedicate this manuscript to the memory of our friend and colleague Omar Antonio Alcaraz-L?pez, whose life has been sadly claimed by the currently ongoing COVID-19 pandemic. This work was funded by the Consejo Nacional de Ciencia y Tecnolog?a, M?xico, through an Institutional Links grant: FONCICYT 277863; and the Birtish Council, through Institutional Links Newton Grant 276305277. The authors wish to thank colleagues at INIFAP, Queretaro for the excellent care and upkeep of the animals used in this experiment and all their help with the collection and processing of samples. The authors also wish to thank Mr Alejandro Nava and Ms Itzel Jim?nez for their help in the handling of the animals at the time of inoculation. BVR and MV are S?r Cymru II Professors at IBERS, AU, funded by the European Research Development Fund and Welsh Government.
Publisher Copyright:
© 2020 Crown copyright. Transboundary and Emerging Diseases published by Wiley-VCH GmbH. This article is published with the permission of the Controller of HMSO and the Queen’s Printer for Scotland.
PY - 2021/11/19
Y1 - 2021/11/19
N2 - Mycobacterium bovis is the main cause of bovine tuberculosis (BTB) in cattle and can also infect humans. Zebu cattle are considered more resistant to some infectious diseases compared with Holstein-Friesian (HF) cattle, including BTB. However, epidemiological studies may not take into account usage differences of the two types of cattle. HF cattle may suffer greater metabolic stress due to their more or less exclusive dairy use, whereas Zebu cattle are mainly used for beef production. In experiments conducted so far, the number of animals has been too small to draw statistically robust conclusions on the resistance differences between these cattle breeds. Here, we used a BCG challenge model to compare the ability of naïve and vaccinated Zebu and HF cattle to control/kill mycobacteria. Young cattle of both breeds with similar ages were housed in the same accommodation for the duration of the experiment. After correcting for multiple comparisons, we found no difference between naïve HF and Zebu (ρ = 0.862) cattle. However, there was a trend for vaccinated HF cattle to have lower cfu numbers than non-vaccinated HF cattle (ρ = 0.057); no such trend was observed between vaccinated and non-vaccinated Zebu cattle (ρ = 0.560). Evaluation of antigen-specific IFNγ secretion by PBMC indicated that Zebu and HF cattle differed in their response to mycobacteria. Thus, whilst there may be difference in immune responses, our data indicate that with the number of animals included in the study and under the conditions used in this work, we were unable to measure any differences between Zebu and HF cattle in the overall control of mycobacteria. Whilst determination of different susceptibilities between Zebu and HF cattle using the BCG challenge model will require larger numbers of animals than the number of animals used in this experiment, these data should inform future experiments.
AB - Mycobacterium bovis is the main cause of bovine tuberculosis (BTB) in cattle and can also infect humans. Zebu cattle are considered more resistant to some infectious diseases compared with Holstein-Friesian (HF) cattle, including BTB. However, epidemiological studies may not take into account usage differences of the two types of cattle. HF cattle may suffer greater metabolic stress due to their more or less exclusive dairy use, whereas Zebu cattle are mainly used for beef production. In experiments conducted so far, the number of animals has been too small to draw statistically robust conclusions on the resistance differences between these cattle breeds. Here, we used a BCG challenge model to compare the ability of naïve and vaccinated Zebu and HF cattle to control/kill mycobacteria. Young cattle of both breeds with similar ages were housed in the same accommodation for the duration of the experiment. After correcting for multiple comparisons, we found no difference between naïve HF and Zebu (ρ = 0.862) cattle. However, there was a trend for vaccinated HF cattle to have lower cfu numbers than non-vaccinated HF cattle (ρ = 0.057); no such trend was observed between vaccinated and non-vaccinated Zebu cattle (ρ = 0.560). Evaluation of antigen-specific IFNγ secretion by PBMC indicated that Zebu and HF cattle differed in their response to mycobacteria. Thus, whilst there may be difference in immune responses, our data indicate that with the number of animals included in the study and under the conditions used in this work, we were unable to measure any differences between Zebu and HF cattle in the overall control of mycobacteria. Whilst determination of different susceptibilities between Zebu and HF cattle using the BCG challenge model will require larger numbers of animals than the number of animals used in this experiment, these data should inform future experiments.
KW - BCG
KW - Holstein-Friesian cattle
KW - mycobacteria
KW - natural resistance
KW - vaccine
KW - Zebu cattle
UR - http://www.scopus.com/inward/record.url?scp=85106806654&partnerID=8YFLogxK
U2 - 10.1111/tbed.13939
DO - 10.1111/tbed.13939
M3 - Article
C2 - 33249779
AN - SCOPUS:85106806654
SN - 1865-1674
VL - 68
SP - 3360
EP - 3365
JO - Transboundary and Emerging Diseases
JF - Transboundary and Emerging Diseases
IS - 6
M1 - 13939
ER -