ATP depletion plays a pivotal role in self-incompatibility, revealing a link between cellular energy status, cytosolic acidification and actin remodelling in pollen tubes

Ludi Wang, Zongcheng Lin, Jose Carli, Agnieszka Gladala-Kostarz, Julia Davies, Vernonica E. Franklin-Tong, Maurice Bosch

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

7 Dyfyniadau(SciVal)
66 Wedi eu Llwytho i Lawr (Pure)

Crynodeb

Self-incompatibility (SI) involves specific interactions during pollination to reject incompatible (‘self’) pollen, preventing inbreeding in angiosperms. A key event observed in pollen undergoing the Papaver rhoeas SI response is the formation of punctate F-actin foci.

Pollen tube growth is heavily energy-dependent, yet ATP levels in pollen tubes have not been directly measured during SI. Here we used transgenic Arabidopsis lines expressing the Papaver pollen S-determinant to investigate a possible link between ATP levels, cytosolic pH ([pH]cyt) and alterations to the actin cytoskeleton.

We identify for the first time that SI triggers a rapid and significant ATP depletion in pollen tubes. Artificial depletion of ATP triggered cytosolic acidification and formation of actin aggregates. We also identify in vivo, evidence for a threshold [pH]cyt of 5.8 for actin foci formation. Imaging revealed that SI stimulates acidic cytosolic patches adjacent to the plasma membrane.

In conclusion, this study provides evidence that ATP depletion plays a pivotal role in SI upstream of programmed cell death and reveals a link between the cellular energy status, cytosolic acidification and alterations to the actin cytoskeleton in regulating Papaver SI in pollen tubes.
Iaith wreiddiolSaesneg
Tudalennau (o-i)1691-1707
Nifer y tudalennau17
CyfnodolynNew Phytologist
Cyfrol236
Rhif cyhoeddi5
Dyddiad ar-lein cynnar23 Gorff 2022
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 03 Tach 2022

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