Biolistics transformation of wheat

Caroline A. Sparks, Huw D Jones

Allbwn ymchwil: Pennod mewn Llyfr/Adroddiad/Trafodion CynhadleddPennod

43 Dyfyniadau (Scopus)

Crynodeb

We present a complete, step-by-step guide to the production of transformed wheat plants using a particle bombardment device to deliver plasmid DNA into immature embryos and the regeneration of transgenic plants via somatic embryogenesis. Currently, this is the most commonly used method for transforming wheat and it offers some advantages. However, it will be interesting to see whether this position is challenged as facile methods are developed for delivering DNA by Agrobacterium tumefaciens or by the production of transformants via a germ-line process (see other chapters in this book).

Iaith wreiddiolSaesneg
TeitlTransgenic Wheat, Barley and Oats
Is-deitlProduction and Characterization Protocols
GolygyddionHuw D. Jones, Peter R. Shewry
CyhoeddwrSpringer Nature
Tudalennau71-92
Nifer y tudalennau22
Cyfrol478
ISBN (Electronig)978-1-59745-379-0
ISBN (Argraffiad)978-1-58829-961-1
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 2009

Cyfres gyhoeddiadau

EnwMethods in Molecular Biology
CyhoeddwrHumana Press
Cyfrol478
ISSN (Argraffiad)1064-3745

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