Buwchitin: A Ruminal Peptide with Antimicrobial Potential against Enterococcus faecalis

Linda Boniface Oyama, Jean-Adrien Crochet, Joan Elizabeth Edwards, Susan Girdwood, Alan Cookson, Narcis Fernandez-Fuentes, Kai Hilpert, Peter N. Golyshin, Olga V. Golyshina, Florence Privé, Matthias Hess, Hilario C. Mantovani, Christopher Creevey, Sharon Huws

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

15 Dyfyniadau (Scopus)
182 Wedi eu Llwytho i Lawr (Pure)

Crynodeb

Antimicrobial peptides (AMPs) are gaining popularity as alternatives for treatment of bacterial infections and recent advances in omics technologies provide new platforms for AMP discovery. We sought to determine the antibacterial activity of a novel antimicrobial peptide, buwchitin, against Enterococcus faecalis. Buwchitin was identified from a rumen bacterial metagenome library, cloned, expressed and purified. The antimicrobial activity of the recombinant peptide was assessed using a broth microdilution susceptibility assay to determine the peptide's killing kinetics against selected bacterial strains. The killing mechanism of buwchitin was investigated further by monitoring its ability to cause membrane depolarization (diSC3(5) method) and morphological changes in E. faecalis cells. Transmission electron micrographs of buwchitin treated E. faecalis cells showed intact outer membranes with blebbing, but no major damaging effects and cell morphology changes. Buwchitin had negligible cytotoxicity against defibrinated sheep erythrocytes. Although no significant membrane leakage and depolarization was observed, buwchitin at minimum inhibitory concentration (MIC) was bacteriostatic against E. faecalis cells and inhibited growth in vitro by 70% when compared to untreated cells. These findings suggest that buwchitin, a rumen derived peptide, has potential for antimicrobial activity against E. faecalis
Iaith wreiddiolSaesneg
Rhif yr erthygl51
CyfnodolynFrontiers in Chemistry
Cyfrol5
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 12 Gorff 2017

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