TY - JOUR
T1 - Candida northwykensis sp nov., A Novel Yeast Isolated from the Gut of the Click Beetle Melanotus villosus
AU - Ravella, Sreenivas Rao
AU - Donovan, Neil
AU - James, Stephen A.
AU - Shivaji, Sisinthy
AU - Arunasri, Kotakonda
AU - Bond, Christopher J.
AU - Roberts, Ian N.
AU - Hobbs, Phil J.
PY - 2011/8
Y1 - 2011/8
N2 - Two yeast morphotypes, BET 4(T) and BET 7, were isolated from the gut of click beetle Melanotus villosus. Click beetles were collected from the decaying timber within the woodlands of North Wyke Research, South West England, UK (latitude, 50A degrees 46'29aEuro(3)N; longitude, 3A degrees 55'23aEuro(3)W). Morphotype BET 7 was identified as Debaryomyces hansenii, and the other morphotype, BET 4(T), was found to differs from Priceomyces castillae and Priceomyces haplophilus, its closest phylogenetic neighbours, by 5.0% with respect to the nucleotide sequence of the D1/D2 domain of the large-subunit (LSU) rRNA gene, and by 8.0% with respect to the ribosomal internal-transcribed spacer (ITS) region. BET 4(T) also differ from P. castillae and P. haplophilus in a number of different phenotypic characteristics. Thus, based on the unique nucleotide sequences of its D1/D2 domain and ITS region, its physiological characteristics and an inability to sporulate, strain BET 4(T) is assigned the status of a new species of Candida, for which the name Candida northwykensis sp. nov., is proposed. The type strain is BET 4(T) (NCYC 3525(T) = CBS 11370(T)).
AB - Two yeast morphotypes, BET 4(T) and BET 7, were isolated from the gut of click beetle Melanotus villosus. Click beetles were collected from the decaying timber within the woodlands of North Wyke Research, South West England, UK (latitude, 50A degrees 46'29aEuro(3)N; longitude, 3A degrees 55'23aEuro(3)W). Morphotype BET 7 was identified as Debaryomyces hansenii, and the other morphotype, BET 4(T), was found to differs from Priceomyces castillae and Priceomyces haplophilus, its closest phylogenetic neighbours, by 5.0% with respect to the nucleotide sequence of the D1/D2 domain of the large-subunit (LSU) rRNA gene, and by 8.0% with respect to the ribosomal internal-transcribed spacer (ITS) region. BET 4(T) also differ from P. castillae and P. haplophilus in a number of different phenotypic characteristics. Thus, based on the unique nucleotide sequences of its D1/D2 domain and ITS region, its physiological characteristics and an inability to sporulate, strain BET 4(T) is assigned the status of a new species of Candida, for which the name Candida northwykensis sp. nov., is proposed. The type strain is BET 4(T) (NCYC 3525(T) = CBS 11370(T)).
KW - INSECTS
KW - GREEN LACEWINGS
KW - COLEOPTERA
KW - CERAMBYCIDAE
KW - SEQUENCE ALIGNMENT
KW - WOOD
UR - http://hdl.handle.net/2160/9087
U2 - 10.1007/s00284-011-9952-y
DO - 10.1007/s00284-011-9952-y
M3 - Article
SN - 0343-8651
VL - 63
SP - 115
EP - 120
JO - Current Microbiology
JF - Current Microbiology
IS - 2
ER -