Prosiectau fesul blwyddyn
The Arabidopsis cyclin-dependent kinase G (CDKG) gene defines a clade of cyclin-dependent protein kinases related to CDK10 and CDK11, as well as to the enigmatic Ph1-related kinases that are implicated in controlling homeologous chromosome pairing in wheat. Here we demonstrate that the CDKG1/CYCLINL complex is essential for synapsis and recombination during male meiosis. A transfer-DNA insertional mutation in the cdkg1 gene leads to a temperature-sensitive failure of meiosis in late Zygotene/Pachytene that is associated with defective formation of the synaptonemal complex, reduced bivalent formation and crossing over, and aneuploid gametes. An aphenotypic insertion in the cyclin L gene, a cognate cyclin for CDKG, strongly enhances the phenotype of cdkg1-1 mutants, indicating that this cdk-cyclin complex is essential for male meiosis. Since CYCLINL, CDKG, and their mammalian homologs have been previously shown to affect mRNA processing, particularly alternative splicing, our observations also suggest a mechanism to explain the widespread phenomenon of thermal sensitivity in male meiosis.
|Nifer y tudalennau||6|
|Cyfnodolyn||Proceedings of the National Academy of Sciences of the United States of America|
|Dynodwyr Gwrthrych Digidol (DOIs)|
|Statws||Cyhoeddwyd - 11 Chwef 2014|
Ôl bysGweld gwybodaeth am bynciau ymchwil 'CDKG1 protein kinase is essential for synapsis and male meiosis at high ambient temperature in Arabidopsis thaliana'. Gyda’i gilydd, maen nhw’n ffurfio ôl bys unigryw.
- Cyfadran Gwyddorau Daear a Bywyd, Athrofa y Gwyddorau Biolegol, Amgylcheddol a Gwledig - Chair (DLM)
Unigolyn: Dysgu ac Ymchwil
- 2 Wedi Gorffen
06 Hyd 2008 → 05 Hyd 2011
Prosiect: Ymchwil a ariannwyd yn allanol