Characterisation of complex formation between members of the Mycobacterium tuberculosis complex CFP-10/ESAT-6 protein family: Towards an understanding of the rules governing complex formation and thereby functional flexibility

Kirsty L. Lightbody; Philip S. Renshaw; Michelle L. Collins; Rebecca L. Wright; Debbie M. Hunt; Stephen V. Gordon; R. Glyn Hewinson; Roger S. Buxton; Richard A. Williamson; Mark D. Carr

doi:10.1111/j.1574-6968.2004.tb09764.x

Characterisation of complex formation between members of the Mycobacterium tuberculosis complex CFP-10/ESAT-6 protein family: Towards an understanding of the rules governing complex formation and thereby functional flexibility

Kirsty L. Lightbody, Philip S. Renshaw, Michelle L. Collins, Rebecca L. Wright, Debbie M. Hunt, Stephen V. Gordon, R. Glyn Hewinson, Roger S. Buxton, Richard A. Williamson, Mark D. Carr

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We have previously shown that the secreted M. tuberculosis complex proteins CFP-10 and ESAT-6 form a tight, 1:1 complex, which may represent their functional form. In the work reported here a combination of yeast two-hybrid and biochemical analysis has been used to characterise complex formation between two other pairs of CFP-10/ESAT-6 family proteins (Rv0287/Rv0288 and Rv3019c/Rv3020c) and to determine whether complexes can be formed between non-genome paired members of the family. The results clearly demonstrate that Rv0287/Rv0288 and Rv3019c/3020c form tight complexes, as initially observed for CFP-10/ESAT-6. The closely related Rv0287/Rv0288 and Rv3019c/Rv3020c proteins are also able to form non-genome paired complexes (Rv0287/Rv3019c and Rv0288/Rv3020c), but are not capable of binding to the more distantly related CFP-10/ESAT-6 proteins.

Iaith wreiddiol	Saesneg
Tudalennau (o-i)	255-262
Nifer y tudalennau	8
Cyfnodolyn	FEMS Microbiology Letters
Cyfrol	238
Rhif cyhoeddi	1
Dyddiad ar-lein cynnar	29 Gorff 2004
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1111/j.1574-6968.2004.tb09764.x
Statws	Cyhoeddwyd - 01 Medi 2004

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Mae’r allbwn hwn yn cyfrannu at y Nod(au) Datblygu Cynaliadwy canlynol

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10.1111/j.1574-6968.2004.tb09764.x

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Gweld gwybodaeth am bynciau ymchwil 'Characterisation of complex formation between members of the Mycobacterium tuberculosis complex CFP-10/ESAT-6 protein family: Towards an understanding of the rules governing complex formation and thereby functional flexibility'. Gyda’i gilydd, maen nhw’n ffurfio ôl bys unigryw.

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Lightbody, K. L., Renshaw, P. S., Collins, M. L., Wright, R. L., Hunt, D. M., Gordon, S. V., Hewinson, R. G., Buxton, R. S., Williamson, R. A., & Carr, M. D. (2004). Characterisation of complex formation between members of the Mycobacterium tuberculosis complex CFP-10/ESAT-6 protein family: Towards an understanding of the rules governing complex formation and thereby functional flexibility. FEMS Microbiology Letters, 238(1), 255-262. https://doi.org/10.1111/j.1574-6968.2004.tb09764.x

Lightbody, Kirsty L. ; Renshaw, Philip S. ; Collins, Michelle L. et al. / Characterisation of complex formation between members of the Mycobacterium tuberculosis complex CFP-10/ESAT-6 protein family : Towards an understanding of the rules governing complex formation and thereby functional flexibility. Yn: FEMS Microbiology Letters. 2004 ; Cyfrol 238, Rhif 1. tt. 255-262.

@article{e183a2592ecc47f984ada60ddbdebe01,

title = "Characterisation of complex formation between members of the Mycobacterium tuberculosis complex CFP-10/ESAT-6 protein family: Towards an understanding of the rules governing complex formation and thereby functional flexibility",

abstract = "We have previously shown that the secreted M. tuberculosis complex proteins CFP-10 and ESAT-6 form a tight, 1:1 complex, which may represent their functional form. In the work reported here a combination of yeast two-hybrid and biochemical analysis has been used to characterise complex formation between two other pairs of CFP-10/ESAT-6 family proteins (Rv0287/Rv0288 and Rv3019c/Rv3020c) and to determine whether complexes can be formed between non-genome paired members of the family. The results clearly demonstrate that Rv0287/Rv0288 and Rv3019c/3020c form tight complexes, as initially observed for CFP-10/ESAT-6. The closely related Rv0287/Rv0288 and Rv3019c/Rv3020c proteins are also able to form non-genome paired complexes (Rv0287/Rv3019c and Rv0288/Rv3020c), but are not capable of binding to the more distantly related CFP-10/ESAT-6 proteins.",

keywords = "CFP-10, ESAT-6, ESAT-6 family members, Protein-protein interaction, Tuberculosis",

author = "Lightbody, {Kirsty L.} and Renshaw, {Philip S.} and Collins, {Michelle L.} and Wright, {Rebecca L.} and Hunt, {Debbie M.} and Gordon, {Stephen V.} and Hewinson, {R. Glyn} and Buxton, {Roger S.} and Williamson, {Richard A.} and Carr, {Mark D.}",

year = "2004",

month = sep,

day = "1",

doi = "10.1111/j.1574-6968.2004.tb09764.x",

language = "English",

volume = "238",

pages = "255--262",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Wiley",

number = "1",

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Lightbody, KL, Renshaw, PS, Collins, ML, Wright, RL, Hunt, DM, Gordon, SV, Hewinson, RG, Buxton, RS, Williamson, RA & Carr, MD 2004, 'Characterisation of complex formation between members of the Mycobacterium tuberculosis complex CFP-10/ESAT-6 protein family: Towards an understanding of the rules governing complex formation and thereby functional flexibility', FEMS Microbiology Letters, cyfrol. 238, rhif 1, tt. 255-262. https://doi.org/10.1111/j.1574-6968.2004.tb09764.x

Characterisation of complex formation between members of the Mycobacterium tuberculosis complex CFP-10/ESAT-6 protein family: Towards an understanding of the rules governing complex formation and thereby functional flexibility. / Lightbody, Kirsty L.; Renshaw, Philip S.; Collins, Michelle L. et al.
Yn: FEMS Microbiology Letters, Cyfrol 238, Rhif 1, 01.09.2004, t. 255-262.

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

TY - JOUR

T1 - Characterisation of complex formation between members of the Mycobacterium tuberculosis complex CFP-10/ESAT-6 protein family

T2 - Towards an understanding of the rules governing complex formation and thereby functional flexibility

AU - Lightbody, Kirsty L.

AU - Renshaw, Philip S.

AU - Collins, Michelle L.

AU - Wright, Rebecca L.

AU - Hunt, Debbie M.

AU - Gordon, Stephen V.

AU - Hewinson, R. Glyn

AU - Buxton, Roger S.

AU - Williamson, Richard A.

AU - Carr, Mark D.

PY - 2004/9/1

Y1 - 2004/9/1

N2 - We have previously shown that the secreted M. tuberculosis complex proteins CFP-10 and ESAT-6 form a tight, 1:1 complex, which may represent their functional form. In the work reported here a combination of yeast two-hybrid and biochemical analysis has been used to characterise complex formation between two other pairs of CFP-10/ESAT-6 family proteins (Rv0287/Rv0288 and Rv3019c/Rv3020c) and to determine whether complexes can be formed between non-genome paired members of the family. The results clearly demonstrate that Rv0287/Rv0288 and Rv3019c/3020c form tight complexes, as initially observed for CFP-10/ESAT-6. The closely related Rv0287/Rv0288 and Rv3019c/Rv3020c proteins are also able to form non-genome paired complexes (Rv0287/Rv3019c and Rv0288/Rv3020c), but are not capable of binding to the more distantly related CFP-10/ESAT-6 proteins.

AB - We have previously shown that the secreted M. tuberculosis complex proteins CFP-10 and ESAT-6 form a tight, 1:1 complex, which may represent their functional form. In the work reported here a combination of yeast two-hybrid and biochemical analysis has been used to characterise complex formation between two other pairs of CFP-10/ESAT-6 family proteins (Rv0287/Rv0288 and Rv3019c/Rv3020c) and to determine whether complexes can be formed between non-genome paired members of the family. The results clearly demonstrate that Rv0287/Rv0288 and Rv3019c/3020c form tight complexes, as initially observed for CFP-10/ESAT-6. The closely related Rv0287/Rv0288 and Rv3019c/Rv3020c proteins are also able to form non-genome paired complexes (Rv0287/Rv3019c and Rv0288/Rv3020c), but are not capable of binding to the more distantly related CFP-10/ESAT-6 proteins.

KW - CFP-10

KW - ESAT-6

KW - ESAT-6 family members

KW - Protein-protein interaction

KW - Tuberculosis

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U2 - 10.1111/j.1574-6968.2004.tb09764.x

DO - 10.1111/j.1574-6968.2004.tb09764.x

M3 - Article

C2 - 15336430

AN - SCOPUS:4444243999

SN - 0378-1097

VL - 238

SP - 255

EP - 262

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 1

ER -

Lightbody KL, Renshaw PS, Collins ML, Wright RL, Hunt DM, Gordon SV et al. Characterisation of complex formation between members of the Mycobacterium tuberculosis complex CFP-10/ESAT-6 protein family: Towards an understanding of the rules governing complex formation and thereby functional flexibility. FEMS Microbiology Letters. 2004 Medi 1;238(1):255-262. Epub 2004 Gor 29. doi: 10.1111/j.1574-6968.2004.tb09764.x