TY - JOUR
T1 - Clostridium proteoclasticum: a ruminal bacterium that forms stearic acid from linoleic acid
AU - Paillard, Delphine
AU - Richardson, Anthony J.
AU - Walker, Nicola D.
AU - McKain, Nest
AU - McEwan, Neil R.
AU - Wallace, R. John
AU - Vercoe, Philip E.
AU - Chaudhary, Lal C.
N1 - Wallace, R. J., Chaudhary, L. C., McKain, N., McEwan, N. R., Richardson, A. J., Vercoe, P. E., Walker, N. D., Paillard, D. (2006). Clostridium proteoclasticum: a ruminal bacterium that forms stearic acid from linoleic acid. FEMS Microbiology Letters, 265, (2), 195-201.
Sponsorship: Scottish Executive Environmental and Rural Affairs Department
PY - 2006/10/18
Y1 - 2006/10/18
N2 - The aim of this study was to identify ruminal bacteria that form stearic acid (18 : 0)
from linoleic acid (cis-9,cis-12-18 : 2). One 18 : 0-producing isolate, P-18, isolated
from the sheep rumen was similar in morphology and metabolic properties to
‘Fusocillus’ spp. isolated many years ago. Phylogenetic analysis based on nearly fulllength
16S rRNA gene sequence (41300 bp) analysis indicated that the stearate
producer was most closely related to Clostridium proteoclasticum B316T. Clostridium
proteoclasticum B316T was also found to form 18 : 0, as were other bacteria
isolated elsewhere, which occurred in the same family subclass of the low G1C%
Gram-positive bacteria, related to Butyrivibrio fibrisolvens. These bacteria are not
clostridia, and the ability to form 18 : 0 was present in all strains in contrast to
proteolytic activity, which was variable. Production of 18 : 0 occurred in growing,
but not in stationary-phase, bacteria, which made detection of biohydrogenating
activity difficult, because of the inhibitory effects of linoleic acid on growth.
AB - The aim of this study was to identify ruminal bacteria that form stearic acid (18 : 0)
from linoleic acid (cis-9,cis-12-18 : 2). One 18 : 0-producing isolate, P-18, isolated
from the sheep rumen was similar in morphology and metabolic properties to
‘Fusocillus’ spp. isolated many years ago. Phylogenetic analysis based on nearly fulllength
16S rRNA gene sequence (41300 bp) analysis indicated that the stearate
producer was most closely related to Clostridium proteoclasticum B316T. Clostridium
proteoclasticum B316T was also found to form 18 : 0, as were other bacteria
isolated elsewhere, which occurred in the same family subclass of the low G1C%
Gram-positive bacteria, related to Butyrivibrio fibrisolvens. These bacteria are not
clostridia, and the ability to form 18 : 0 was present in all strains in contrast to
proteolytic activity, which was variable. Production of 18 : 0 occurred in growing,
but not in stationary-phase, bacteria, which made detection of biohydrogenating
activity difficult, because of the inhibitory effects of linoleic acid on growth.
M3 - Article
SN - 0378-1097
SP - 195
EP - 201
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
ER -