Combinatorial labelling of DNA probes enables multicolour fluorescence in situ hybridisation in plants

Robert Hasterok; Tim Langdon; Steve Taylor; Glyn M. Jenkins

Combinatorial labelling of DNA probes enables multicolour fluorescence in situ hybridisation in plants

Robert Hasterok, Tim Langdon, Steve Taylor, Glyn M. Jenkins

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Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

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This paper demonstrates a simple but effective use of combinatorial probes to label plant chromosomes by multicolour fluorescence in situ hybridisation (FISH). Three different DNA probes were labelled with only two different fluorophores, hybridised to somatic metaphase chromosomes of Secale cereale and Triticum aestivum, simultaneously visualised, and unequivocally distinguished in a single FISH experiment. Combinatorial labelling can augment karyotypical investigations, physical mapping of chromosomes and other analyses in plants based upon FISH.

Iaith wreiddiol	Saesneg
Tudalennau (o-i)	319-323
Nifer y tudalennau	5
Cyfnodolyn	Folia Histochemica et Cytobiologica
Cyfrol	40
Rhif cyhoeddi	3
Statws	Cyhoeddwyd - 2002

Mynediad at Ddogfen

Cysylltiad parhaol

Dolen barhaus

Dyfynnu hyn

@article{18024bc6a8ac4b2e96338215083fa790,

title = "Combinatorial labelling of DNA probes enables multicolour fluorescence in situ hybridisation in plants",

abstract = "This paper demonstrates a simple but effective use of combinatorial probes to label plant chromosomes by multicolour fluorescence in situ hybridisation (FISH). Three different DNA probes were labelled with only two different fluorophores, hybridised to somatic metaphase chromosomes of Secale cereale and Triticum aestivum, simultaneously visualised, and unequivocally distinguished in a single FISH experiment. Combinatorial labelling can augment karyotypical investigations, physical mapping of chromosomes and other analyses in plants based upon FISH.",

author = "Robert Hasterok and Tim Langdon and Steve Taylor and Jenkins, {Glyn M.}",

note = "Hasterok, R., Langdon, T., Taylor, S., Jones, R. N., Jenkins, G. (2002). Combinatorial labelling of DNA probes enables multicolour fluorescence in situ hybridisation in plants. Folia Histochemica et Cytobiologica, 40 (3), 319-323.",

year = "2002",

language = "English",

volume = "40",

pages = "319--323",

journal = "Folia Histochemica et Cytobiologica",

issn = "1897-5631",

publisher = "Polish Histochemical and Cytochemical Society",

number = "3",

}

TY - JOUR

T1 - Combinatorial labelling of DNA probes enables multicolour fluorescence in situ hybridisation in plants

AU - Hasterok, Robert

AU - Langdon, Tim

AU - Taylor, Steve

AU - Jenkins, Glyn M.

N1 - Hasterok, R., Langdon, T., Taylor, S., Jones, R. N., Jenkins, G. (2002). Combinatorial labelling of DNA probes enables multicolour fluorescence in situ hybridisation in plants. Folia Histochemica et Cytobiologica, 40 (3), 319-323.

PY - 2002

Y1 - 2002

N2 - This paper demonstrates a simple but effective use of combinatorial probes to label plant chromosomes by multicolour fluorescence in situ hybridisation (FISH). Three different DNA probes were labelled with only two different fluorophores, hybridised to somatic metaphase chromosomes of Secale cereale and Triticum aestivum, simultaneously visualised, and unequivocally distinguished in a single FISH experiment. Combinatorial labelling can augment karyotypical investigations, physical mapping of chromosomes and other analyses in plants based upon FISH.

AB - This paper demonstrates a simple but effective use of combinatorial probes to label plant chromosomes by multicolour fluorescence in situ hybridisation (FISH). Three different DNA probes were labelled with only two different fluorophores, hybridised to somatic metaphase chromosomes of Secale cereale and Triticum aestivum, simultaneously visualised, and unequivocally distinguished in a single FISH experiment. Combinatorial labelling can augment karyotypical investigations, physical mapping of chromosomes and other analyses in plants based upon FISH.

M3 - Article

SN - 1897-5631

VL - 40

SP - 319

EP - 323

JO - Folia Histochemica et Cytobiologica

JF - Folia Histochemica et Cytobiologica

IS - 3

ER -

Combinatorial labelling of DNA probes enables multicolour fluorescence in situ hybridisation in plants

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