Prosiectau fesul blwyddyn
Crynodeb
Manual phenotyping of rice tillers is time consuming and labor intensive, and lags behind the rapid development of rice functional genomics. Thus, automated, non-destructive methods of phenotyping rice tiller traits at a high spatial resolution and high throughput for large-scale assessment of rice accessions are urgently needed. In this study, we developed a high-throughput micro-CT-RGB imaging system to non-destructively extract 739 traits from 234 rice accessions at nine time points. We could explain 30% of the grain yield variance from two tiller traits assessed in the early growth stages. A total of 402 significantly associated loci were identified by genome-wide association study, and dynamic and static genetic components were found across the nine time points. A major locus associated with tiller angle was detected at time point 9, which contained a major gene, TAC1. Significant variants associated with tiller angle were enriched in the 3ʹ-untranslated region of TAC1. Three haplotypes for the gene were found, and rice accessions containing haplotype H3 displayed much smaller tiller angles. Further, we found two loci containing associations with both vigor-related traits identified by high-throughput micro-CT-RGB imaging and yield. The superior alleles would be beneficial for breeding for high yield and dense planting.
Iaith wreiddiol | Saesneg |
---|---|
Tudalennau (o-i) | 545-561 |
Nifer y tudalennau | 17 |
Cyfnodolyn | Journal of Experimental Botany |
Cyfrol | 70 |
Rhif cyhoeddi | 2 |
Dyddiad ar-lein cynnar | 30 Hyd 2018 |
Dynodwyr Gwrthrych Digidol (DOIs) | |
Statws | Cyhoeddwyd - 07 Ion 2019 |
Ôl bys
Gweld gwybodaeth am bynciau ymchwil 'Combining high-throughput micro-CT-RGB phenotyping and genome-wide association study to dissect the genetic architecture of tiller growth in rice'. Gyda’i gilydd, maen nhw’n ffurfio ôl bys unigryw.Proffiliau
Prosiectau
- 3 Wedi Gorffen
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A China-UK joint phenomics consortium to dissect the basis of crop stress resistance in the face of climate change
Doonan, J. (Prif Ymchwilydd), Han, J. (Cyd-ymchwilydd), Liu, Y. (Cyd-ymchwilydd) & Mur, L. (Cyd-ymchwilydd)
Biotechnology and Biological Sciences Research Council
01 Gorff 2018 → 31 Rhag 2023
Prosiect: Ymchwil a ariannwyd yn allanol
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BBSRC Core Strategic Programme in Resilient Crops: Oats
Howarth, C. (Prif Ymchwilydd)
01 Ebr 2017 → 31 Maw 2022
Prosiect: Ymchwil a ariannwyd yn allanol
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Phenomics National Capability Grant ISPG
Doonan, J. (Prif Ymchwilydd)
Biotechnology and Biological Sciences Research Council
01 Ebr 2012 → 31 Maw 2017
Prosiect: Ymchwil a ariannwyd yn allanol