Comparison of tuberculin activity using the interferon-γ assay for the diagnosis of bovine tuberculosis

I. Schiller; H. M. Vordermeier; W. R. Waters; A. Kyburz; M. Cagiola; A. Whelan; M. V. Palmer; T. C. Thacker; J. Meijlis; C. Carter; S. Gordon; T. Egnuni; R. Hardegger; B. Marg-Haufe; A. Raeber; B. Oesch

doi:10.1136/vr.c3403

Comparison of tuberculin activity using the interferon-γ assay for the diagnosis of bovine tuberculosis

I. Schiller, H. M. Vordermeier, W. R. Waters, A. Kyburz, M. Cagiola, A. Whelan, M. V. Palmer, T. C. Thacker, J. Meijlis, C. Carter, S. Gordon, T. Egnuni, R. Hardegger, B. Marg-Haufe, A. Raeber, B. Oesch^*

^*Awdur cyfatebol y gwaith hwn

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

26 Dyfyniadau (Scopus)

Crynodeb

In this study, interferon-γ (IFN-γ) responses in whole blood cultures stimulated with tuberculins from different sources were compared with regard to their diagnostic reliability in cattle experimentally and naturally infected with Mycobacterium bovis. The IFN-γ responses to different concentrations of purified protein derivatives (PPDs) from M bovis and Mycobacterium avium were quantified. Significant differences (P<0.05) between sources and concentrations of PPDs used for stimulation were detected, indicating a need for standardisation of PPDs used in the IFN-γ assay. Additionally, a tool named 'relative potency 30' that allows rapid comparison of batches and sources of PPDs was defined.

Iaith wreiddiol	Saesneg
Tudalennau (o-i)	322-326
Nifer y tudalennau	5
Cyfnodolyn	Veterinary Record
Cyfrol	167
Rhif cyhoeddi	9
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1136/vr.c3403
Statws	Cyhoeddwyd - 28 Awst 2010

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Mae’r allbwn hwn yn cyfrannu at y Nod(au) Datblygu Cynaliadwy canlynol

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10.1136/vr.c3403

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Link to publication in Scopus

Dyfynnu hyn

Schiller, I., Vordermeier, H. M., Waters, W. R., Kyburz, A., Cagiola, M., Whelan, A., Palmer, M. V., Thacker, T. C., Meijlis, J., Carter, C., Gordon, S., Egnuni, T., Hardegger, R., Marg-Haufe, B., Raeber, A., & Oesch, B. (2010). Comparison of tuberculin activity using the interferon-γ assay for the diagnosis of bovine tuberculosis. Veterinary Record, 167(9), 322-326. https://doi.org/10.1136/vr.c3403

@article{24af4e46a0d34a798afaedef3e8c51ae,

title = "Comparison of tuberculin activity using the interferon-γ assay for the diagnosis of bovine tuberculosis",

abstract = "In this study, interferon-γ (IFN-γ) responses in whole blood cultures stimulated with tuberculins from different sources were compared with regard to their diagnostic reliability in cattle experimentally and naturally infected with Mycobacterium bovis. The IFN-γ responses to different concentrations of purified protein derivatives (PPDs) from M bovis and Mycobacterium avium were quantified. Significant differences (P<0.05) between sources and concentrations of PPDs used for stimulation were detected, indicating a need for standardisation of PPDs used in the IFN-γ assay. Additionally, a tool named 'relative potency 30' that allows rapid comparison of batches and sources of PPDs was defined.",

author = "I. Schiller and Vordermeier, {H. M.} and Waters, {W. R.} and A. Kyburz and M. Cagiola and A. Whelan and Palmer, {M. V.} and Thacker, {T. C.} and J. Meijlis and C. Carter and S. Gordon and T. Egnuni and R. Hardegger and B. Marg-Haufe and A. Raeber and B. Oesch",

year = "2010",

month = aug,

day = "28",

doi = "10.1136/vr.c3403",

language = "English",

volume = "167",

pages = "322--326",

journal = "Veterinary Record",

issn = "0042-4900",

publisher = "Wiley",

number = "9",

}

Schiller, I, Vordermeier, HM, Waters, WR, Kyburz, A, Cagiola, M, Whelan, A, Palmer, MV, Thacker, TC, Meijlis, J, Carter, C, Gordon, S, Egnuni, T, Hardegger, R, Marg-Haufe, B, Raeber, A & Oesch, B 2010, 'Comparison of tuberculin activity using the interferon-γ assay for the diagnosis of bovine tuberculosis', Veterinary Record, cyfrol. 167, rhif 9, tt. 322-326. https://doi.org/10.1136/vr.c3403

TY - JOUR

T1 - Comparison of tuberculin activity using the interferon-γ assay for the diagnosis of bovine tuberculosis

AU - Schiller, I.

AU - Vordermeier, H. M.

AU - Waters, W. R.

AU - Kyburz, A.

AU - Cagiola, M.

AU - Whelan, A.

AU - Palmer, M. V.

AU - Thacker, T. C.

AU - Meijlis, J.

AU - Carter, C.

AU - Gordon, S.

AU - Egnuni, T.

AU - Hardegger, R.

AU - Marg-Haufe, B.

AU - Raeber, A.

AU - Oesch, B.

PY - 2010/8/28

Y1 - 2010/8/28

N2 - In this study, interferon-γ (IFN-γ) responses in whole blood cultures stimulated with tuberculins from different sources were compared with regard to their diagnostic reliability in cattle experimentally and naturally infected with Mycobacterium bovis. The IFN-γ responses to different concentrations of purified protein derivatives (PPDs) from M bovis and Mycobacterium avium were quantified. Significant differences (P<0.05) between sources and concentrations of PPDs used for stimulation were detected, indicating a need for standardisation of PPDs used in the IFN-γ assay. Additionally, a tool named 'relative potency 30' that allows rapid comparison of batches and sources of PPDs was defined.

AB - In this study, interferon-γ (IFN-γ) responses in whole blood cultures stimulated with tuberculins from different sources were compared with regard to their diagnostic reliability in cattle experimentally and naturally infected with Mycobacterium bovis. The IFN-γ responses to different concentrations of purified protein derivatives (PPDs) from M bovis and Mycobacterium avium were quantified. Significant differences (P<0.05) between sources and concentrations of PPDs used for stimulation were detected, indicating a need for standardisation of PPDs used in the IFN-γ assay. Additionally, a tool named 'relative potency 30' that allows rapid comparison of batches and sources of PPDs was defined.

UR - http://www.scopus.com/inward/record.url?scp=78049448061&partnerID=8YFLogxK

U2 - 10.1136/vr.c3403

DO - 10.1136/vr.c3403

M3 - Article

C2 - 20802185

AN - SCOPUS:78049448061

SN - 0042-4900

VL - 167

SP - 322

EP - 326

JO - Veterinary Record

JF - Veterinary Record

IS - 9

ER -

Comparison of tuberculin activity using the interferon-γ assay for the diagnosis of bovine tuberculosis

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