Construction of high-resolution comparative maps in mammals using BAC-end sequences

Denis Larkin, Harris Lewin

Allbwn ymchwil: Pennod mewn Llyfr/Adroddiad/Trafodion CynhadleddPennod

1 Dyfyniad (Scopus)

Crynodeb

Whole-genome bacterial artificial chromosome (BAC) libraries for ∼40 representative species of mammals provide unique resources for the construction of high-resolution ordered genomic maps, the development of species genetics, and multispecies genome comparisons. Herein we describe procedures to construct high-quality radiation hybrid comparative maps using BAC-end sequences (BESs). This approach has been applied to the construction of ∼1 Mbp resolution porcine-human and cattle-human comparative maps. High-resolution ordered comparative maps built with BESs and whole-genome sequences have been shown to be an invaluable resource for the analysis of mammalian chromosome evolution, and as a backbone for the assembly of whole-genome shotgun sequences.
Iaith wreiddiolSaesneg
TeitlPhylogenomics
GolygyddionWilliam J. Murphy
CyhoeddwrSpringer Nature
Tudalennau79-90
Nifer y tudalennau12
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 2008

Cyfres gyhoeddiadau

EnwMethods in Molecular Biology
CyhoeddwrHumana Press
Cyfrol422
ISSN (Argraffiad)1064-3745

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