TY - JOUR
T1 - Cryptococcus shivajii sp nov.: A Novel Basidiomycetous Yeast Isolated from Biogas Reactor
AU - Ravella, Sreenivas Rao
AU - James, Stephen A.
AU - Bond, Christopher J.
AU - Roberts, Ian N.
AU - Cross, Kathryn
AU - Retter, Andy
AU - Hobbs, Phil J.
PY - 2010/1
Y1 - 2010/1
N2 - Five yeast morphotypes were isolated from biogas reactors at North Wyke Research, Okehampton, UK. Out of the five morphotypes, four were identified as known species. In contrast, the fifth morphotype strain, Bio10(T), was found to differ from Bullera dendrophila and Kwoniella mangroviensis, its closest phylogenetic neighbours, by 2.6-2.9% with respect to the nucleotide sequence of the D1/D2 domain of the 26S rRNA gene and by 5.6-6.2% with respect to the internal transcribed spacer 1 (ITS1)-5.8S rRNA gene-ITS2 region. Bio10(T) also differs from these two species by a number of phenotypic characteristics. Thus, based on the phenotypic differences and phylogenetic analysis, strain Bio10(T) is assigned the status of a new species of Cryptococcus, for which the name Cryptococcus shivajii sp. nov. is proposed. The type strain is Bio10(T) (NCYC 3541(T) = CBS 11374(T)).
AB - Five yeast morphotypes were isolated from biogas reactors at North Wyke Research, Okehampton, UK. Out of the five morphotypes, four were identified as known species. In contrast, the fifth morphotype strain, Bio10(T), was found to differ from Bullera dendrophila and Kwoniella mangroviensis, its closest phylogenetic neighbours, by 2.6-2.9% with respect to the nucleotide sequence of the D1/D2 domain of the 26S rRNA gene and by 5.6-6.2% with respect to the internal transcribed spacer 1 (ITS1)-5.8S rRNA gene-ITS2 region. Bio10(T) also differs from these two species by a number of phenotypic characteristics. Thus, based on the phenotypic differences and phylogenetic analysis, strain Bio10(T) is assigned the status of a new species of Cryptococcus, for which the name Cryptococcus shivajii sp. nov. is proposed. The type strain is Bio10(T) (NCYC 3541(T) = CBS 11374(T)).
KW - FUNGI
KW - ARCHAEA
KW - COMMUNITY
KW - POLYMERASE-CHAIN-REACTION
KW - IDENTIFICATION
KW - DIVERSITY
KW - CONFORMATION POLYMORPHISM ANALYSIS
KW - PHYLOGENIES
KW - ANAEROBIC DIGESTER
UR - http://hdl.handle.net/2160/9086
U2 - 10.1007/s00284-009-9493-9
DO - 10.1007/s00284-009-9493-9
M3 - Article
SN - 0343-8651
VL - 60
SP - 12
EP - 16
JO - Current Microbiology
JF - Current Microbiology
IS - 1
ER -