Prosiectau fesul blwyddyn
Crynodeb
Cell permeating peptides (CPPs) are attracting great interest for use as molecular delivery vehicles for the transport of biologically active cargo across the cell membrane. The sequence of a novel CPP sequence, termed ‘Cupid’, was identified from the genome of Dictyostelium discoideum. A Cupid-Green Fluorescent Protein (Cupid-GFP) fusion protein was tested on mammalian, whole plant cells, plant leaf protoplast and fungal cell cultures and observed using confocal microscopy. GFP fluorescence builds up within the cell cytosol in 60 min, demonstrating Cupid-GFP has permeated them and folded correctly into its fluorescent form. Our combined data suggest Cupid can act as a molecular vehicle capable of delivering proteins, such as GFP, into the cytosol of a variety of cells.
Iaith wreiddiol | Saesneg |
---|---|
Rhif yr erthygl | 13725 |
Nifer y tudalennau | 11 |
Cyfnodolyn | Scientific Reports |
Cyfrol | 10 |
Rhif cyhoeddi | 1 |
Dynodwyr Gwrthrych Digidol (DOIs) | |
Statws | Cyhoeddwyd - 13 Awst 2020 |
Ôl bys
Gweld gwybodaeth am bynciau ymchwil 'Cupid, a cell permeable peptide derived from amoeba, capable of delivering GFP into a diverse range of species'. Gyda’i gilydd, maen nhw’n ffurfio ôl bys unigryw.Proffiliau
-
Huw Jones
- Adran y Gwyddorau Bywyd - Chair in Translational Genomics for Plant Breeding
Unigolyn: Dysgu ac Ymchwil
Prosiectau
- 1 Wedi Gorffen
-
BBSRC Core Strategic Programme in Resilient Crops: Grasslands Gogerddan
Armstead, I. (Prif Ymchwilydd), Donnison, I. (Cyd-ymchwilydd), Jones, H. (Cyd-ymchwilydd), Skot, L. (Cyd-ymchwilydd), Fernandez Fuentes, N. (Cyd-ymchwilydd), Phillips, D. (Prif Ymchwilydd), Kingston-Smith, A. (Cyd-ymchwilydd) & Bosch, M. (Cyd-ymchwilydd)
Biotechnology and Biological Sciences Research Council
01 Ebr 2017 → 31 Maw 2020
Prosiect: Ymchwil a ariannwyd yn allanol