Cupid, a cell permeable peptide derived from amoeba, capable of delivering GFP into a diverse range of species

Daniel Fenton, Dylan Phillips, Anne Maddison, Christopher H. George, Jonathan Ryves, Huw D. Jones*

*Awdur cyfatebol y gwaith hwn

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

5 Dyfyniadau (Scopus)
163 Wedi eu Llwytho i Lawr (Pure)

Crynodeb

Cell permeating peptides (CPPs) are attracting great interest for use as molecular delivery vehicles for the transport of biologically active cargo across the cell membrane. The sequence of a novel CPP sequence, termed ‘Cupid’, was identified from the genome of Dictyostelium discoideum. A Cupid-Green Fluorescent Protein (Cupid-GFP) fusion protein was tested on mammalian, whole plant cells, plant leaf protoplast and fungal cell cultures and observed using confocal microscopy. GFP fluorescence builds up within the cell cytosol in 60 min, demonstrating Cupid-GFP has permeated them and folded correctly into its fluorescent form. Our combined data suggest Cupid can act as a molecular vehicle capable of delivering proteins, such as GFP, into the cytosol of a variety of cells.

Iaith wreiddiolSaesneg
Rhif yr erthygl13725
Nifer y tudalennau11
CyfnodolynScientific Reports
Cyfrol10
Rhif cyhoeddi1
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 13 Awst 2020

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