Cyclophilin A is a new M cell marker of bovine intestinal epithelium

Tetsuya Hondo, Shunsuke Someya, Yuya Nagasawa, Shunsuke Terada, Hitoshi Watanabe, Xiangning Chen, Kouichi Watanabe, Shyuichi Ohwada, Haruki Kitazawa, Michael Rose, Tomonori Nochi, Hisashi Aso

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

3 Dyfyniadau(SciVal)
124 Wedi eu Llwytho i Lawr (Pure)

Crynodeb

Microfold (M) cells in the follicle-associated epithelium (FAE) of Peyer’s patches contribute to the mucosal immune response by the transcytosis of microorganisms. The mechanism by which M cells take up microorganisms, and the functional proteins by which they do this, are not clear. In order to explore one such protein, we developed a 2H5-F3 monoclonal antibody (2H5-F3 mAb) through its binding to bovine M cells, and identified the antibody reactive molecule as cyclophilin A (Cyp-A). The localization patterns of Cyp-A were very similar to the localization pattern of cytokeratin (CK) 18-positive M cells. Cyp-A was identified at the luminal surface of CK18-positive M cells in bovine jejunal and ileal FAE. The membranous localization of Cyp-A in the bovine intestinal cell line (BIE cells) increased as cells differentiated toward M cells, as determined by flow cytometry analysis. Additionally, BIE cells released Cyp-A to the extracellular space and the differentiation of BIE cells to M cells increased the secretion of Cyp-A, as determined by western blotting. Accordingly, Cyp-A may be localized in M cells in the small intestinal epithelium of cattle. The rise of the membranous localization and secretion of Cyp-A by differentiation toward M cells indicates that Cyp-A has an important role in the function of M cells. While Cyp-A of the M cell membrane may contribute to the uptake of viruses with peptidyl-prolyl cis-trans isomerase activity, in the extracellular space Cyp-A may work as a chemokine and contribute to the distribution of immuno-competent cells.
Iaith wreiddiolSaesneg
Tudalennau (o-i)585-597
Nifer y tudalennau13
CyfnodolynCell and Tissue Research
Cyfrol364
Rhif cyhoeddi3
Dyddiad ar-lein cynnar22 Chwef 2016
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 01 Meh 2016

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