Prosiectau fesul blwyddyn
Crynodeb
White clover is a short-lived perennial whose persistence is greatly affected by abiotic stresses, particularly drought. An understanding of its molecular response to water deficit and recovery following re-hydration is of paramount importance to identify targets for the breeding of tolerant varieties.
Results
We created a white clover reference transcriptome of 16,193 contigs by de novo and reference-based assembly of Illumina reads from four SSH libraries (forward and reverse libraries for each treatment) constructed from leaf material of white clover at the onset of drought and recovery. Reads from individual libraries were then mapped to the reference transcriptome and processed comparing expression level data to generate four robust sets of transcripts induced and repressed in the leaves subjected to and recovering from water stress. The differentially expressed transcripts were functionally annotated and mapped to biological processes and pathways.
Conclusions
The work presented in this paper provides a simple pipeline for the processing of data generated by deep sequencing of SSH libraries and the first large scale molecular analysis of the white clover response to drought stress and recovery.
Iaith wreiddiol | Saesneg |
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Rhif yr erthygl | 213 |
Cyfnodolyn | Frontiers in Plant Science |
Cyfrol | 8 |
Dynodwyr Gwrthrych Digidol (DOIs) | |
Statws | Cyhoeddwyd - 23 Chwef 2017 |
Ôl bys
Gweld gwybodaeth am bynciau ymchwil 'Deep sequencing of Suppression Subtractive Hybridisation drought and recovery libraries of the non-model crop Trifolium repens L.'. Gyda’i gilydd, maen nhw’n ffurfio ôl bys unigryw.Prosiectau
- 3 Wedi Gorffen
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Bioinformatics and genomic and phenomic platform development
Armstead, I. (Prif Ymchwilydd), Boyle, R. (Prif Ymchwilydd), Doonan, J. (Prif Ymchwilydd), Fernandez Fuentes, N. (Prif Ymchwilydd), Gay, A. (Prif Ymchwilydd), Hegarty, M. (Prif Ymchwilydd), Huang, L. (Prif Ymchwilydd), Neal, M. (Prif Ymchwilydd), Swain, M. (Prif Ymchwilydd) & Thomas, I. (Prif Ymchwilydd)
01 Ebr 2012 → 31 Maw 2017
Prosiect: Ymchwil a ariannwyd yn allanol
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Introgression and wide hybrid genetics, genomics and germplasm development in Lolium/Festuca (Festulolium) and white clover
Armstead, I. (Prif Ymchwilydd)
01 Ebr 2012 → 31 Maw 2017
Prosiect: Ymchwil a ariannwyd yn allanol
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C3G: Lolium and Trifolium genetics, genomics and germplasm development
Armstead, I. (Prif Ymchwilydd), Jenkins, G. (Prif Ymchwilydd), Marshall, A. (Prif Ymchwilydd), Skot, L. (Prif Ymchwilydd), Thomas, I. (Prif Ymchwilydd) & Thorogood, D. (Prif Ymchwilydd)
01 Ebr 2012 → 31 Maw 2017
Prosiect: Ymchwil a ariannwyd yn allanol