DNA extraction from spider webs

Max Blake, Niall McKeown, Mark Bushell, Paul Shaw

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

10 Dyfyniadau (Scopus)
160 Wedi eu Llwytho i Lawr (Pure)

Crynodeb

Many spider species produce webs that represent a potential non-invasive source of DNA for conservation genetic analysis. Reported here is the successful isolation of target DNA from members of two families (Theraphosidae and Pholcidae) using a standard CTAB phenol–chloroform–isoamyl protocol. The isolated DNA was of sufficient quality to permit routine PCR amplification and sequencing of mtDNA COI fragments of various sizes (maximum 710 bp attempted). This adds to other studies in demonstrating that webbing offers an excellent resource for genetic studies of spiders across families. Applications of the technique include species identification and monitoring, faunistic surveys, population connectivity, subpopulation structuring, and ex situ breeding programs.
Iaith wreiddiolSaesneg
Tudalennau (o-i)219-221
Nifer y tudalennau3
CyfnodolynConservation Genetics Resources
Cyfrol8
Rhif cyhoeddi3
Dyddiad ar-lein cynnar26 Ebr 2016
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 01 Medi 2016

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