Dual metabolomics: A novel approach to understanding plant-pathogen interactions

James William Allwood, Andrew Clarke, Royston Goodacre, Luis A. J. Mur

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

75 Dyfyniadau(SciVal)

Crynodeb

One of the most well-characterised plant pathogenic interactions involves Arabidopsis thaliana and the bacteria Pseudomonas syringae pathovar tomato (Pst). The standard Pst inoculation procedure involves infiltration of large populations of bacteria into plant leaves which means that metabolite changes cannot be readily assigned to the host or pathogen. A plant cell–pathogen co-culture based approach has been developed where the plant and pathogen cells are separated after 12 h of co-culture via differential filtering and centrifugation. Fourier transform infrared (FT-IR) spectroscopy was employed to assess the intracellular metabolomes (metabolic fingerprints) of both host and pathogen and their extruded (extracellular) metabolites (metabolic footprints) under conditions relevant to disease and resistance. We propose that this system will enable the metabolomic profiling of the separated host and pathogen (i.e. ‘dual metabolomics’) and will facilitate the modelling of reciprocal responses.
Iaith wreiddiolSaesneg
Tudalennau (o-i)590-597
Nifer y tudalennau8
CyfnodolynPhytochemistry
Cyfrol71
Rhif cyhoeddi5-6
Dyddiad ar-lein cynnar05 Chwef 2010
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 01 Ebr 2010

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