Early hepatic and peritoneal changes and immune response in goats vaccinated with a recombinant glutathione transferase sigma class and challenged with Fasciola hepatica.

R Zafra, R. A. Pérez-Écija, L. Buffoni, I. L. Pacheco, A. Martínez-Moreno, E. J. Lacourse , Samirah Perally, Peter Michael Brophy, J. Pérez

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

27 Dyfyniadau (Scopus)

Crynodeb

Changes and local immune response were evaluated in the peritoneal cell populations, duodenal lamina propria and liver from goats immunized with recombinant glutathione transferase sigma class (rFhGST-S1) during early stages of infection with Fasciola hepatica. Group 1 (n=7) was unimmunized and uninfected; group 2 (n=10) was immunized with adjuvant Quil A and infected; group 3 (n=10) was immunised with rFhGST-S1 and infected. Three goats from each group were killed at 7-9days post-infection (dpi) to evaluate early changes and immune response. The remaining goats were killed at 15weeks post-infection (wpi). rFhGST-S1 vaccination induced variable response: three goats showed low fluke burden at 15 wpi and two goats showed low hepatic damage at early infection stages. This response was associated to a severe infiltrate of eosinophils in peritoneal fluid and hepatic necrotic foci, high iNOS expression in peritoneal cells and abundant infiltrate of eosinophils surrounding hepatic migrating flukes. T lymphocyte subsets were found in the vicinity of necrotic areas but they were absent in the vicinity of migrating larvae. No significant variation for T cell subsets, except for CD4 and γδ T lymphocytes, that were higher in the Quil A group compared to the rFhGST-S1 group. Expression of IL4 and IFN-γ in the hepatic inflammatory infiltrates was very occasional
Iaith wreiddiolSaesneg
Tudalennau (o-i)602-609
Nifer y tudalennau8
CyfnodolynResearch in Veterinary Science
Cyfrol94
Rhif cyhoeddi3
Dyddiad ar-lein cynnar20 Rhag 2012
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 01 Meh 2013

Ôl bys

Gweld gwybodaeth am bynciau ymchwil 'Early hepatic and peritoneal changes and immune response in goats vaccinated with a recombinant glutathione transferase sigma class and challenged with Fasciola hepatica.'. Gyda’i gilydd, maen nhw’n ffurfio ôl bys unigryw.

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