Effect of dose and route of immunisation on the immune response induced in cattle by heterologous Bacille Calmette-Guerin priming and recombinant adenoviral vector boosting

G. Dean; D. Clifford; S. Gilbert; H. McShane; R. G. Hewinson; H. M. Vordermeier; B. Villarreal-Ramos

doi:10.1016/j.vetimm.2014.01.010

Effect of dose and route of immunisation on the immune response induced in cattle by heterologous Bacille Calmette-Guerin priming and recombinant adenoviral vector boosting

G. Dean, D. Clifford, S. Gilbert, H. McShane, R. G. Hewinson, H. M. Vordermeier, B. Villarreal-Ramos^*

^*Awdur cyfatebol y gwaith hwn

TB buchol, clefydau a rheolaeth

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

Crynodeb

BCG is used experimentally as a vaccine against tuberculosis (TB), induced by Mycobacterium bovis, in cattle (bTB). However, the efficacy of BCG is variable in humans, cattle and guinea pigs. An adenoviral vector expressing Antigen 85A (Ad5Ag85A) has enhanced protection against TB in mice when used in combination with BCG for prime-boost experiments. However, the route of immunisation affects the degree of protection seen. This work examines the immunogenicity of a new vectored vaccine (Ad5-TBF) that expresses Ag85A, Rv0287, Rv0288 and Rv0251c to explore the effects of dose of adenoviral boost and route of inoculation on immunogenicity. We found that 2×10⁹ infectious units (iu) delivered intradermally conferred the most consistent and strongest responses of the different regimes tested.

Iaith wreiddiol	Saesneg
Tudalennau (o-i)	208-213
Nifer y tudalennau	6
Cyfnodolyn	Veterinary Immunology and Immunopathology
Cyfrol	158
Rhif cyhoeddi	3-4
Dyddiad ar-lein cynnar	28 Ion 2014
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1016/j.vetimm.2014.01.010
Statws	Cyhoeddwyd - 15 Ebr 2014

NDC y CU

Mae’r allbwn hwn yn cyfrannu at y Nod(au) Datblygu Cynaliadwy canlynol

Mynediad at Ddogfen

10.1016/j.vetimm.2014.01.010

Cysylltiad parhaol

Dolen barhaus

Ffeiliau eraill a chysylltiadau

Link to publication in Scopus

Dyfynnu hyn

Dean, G., Clifford, D., Gilbert, S., McShane, H., Hewinson, R. G., Vordermeier, H. M., & Villarreal-Ramos, B. (2014). Effect of dose and route of immunisation on the immune response induced in cattle by heterologous Bacille Calmette-Guerin priming and recombinant adenoviral vector boosting. Veterinary Immunology and Immunopathology, 158(3-4), 208-213. https://doi.org/10.1016/j.vetimm.2014.01.010

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title = "Effect of dose and route of immunisation on the immune response induced in cattle by heterologous Bacille Calmette-Guerin priming and recombinant adenoviral vector boosting",

abstract = "BCG is used experimentally as a vaccine against tuberculosis (TB), induced by Mycobacterium bovis, in cattle (bTB). However, the efficacy of BCG is variable in humans, cattle and guinea pigs. An adenoviral vector expressing Antigen 85A (Ad5Ag85A) has enhanced protection against TB in mice when used in combination with BCG for prime-boost experiments. However, the route of immunisation affects the degree of protection seen. This work examines the immunogenicity of a new vectored vaccine (Ad5-TBF) that expresses Ag85A, Rv0287, Rv0288 and Rv0251c to explore the effects of dose of adenoviral boost and route of inoculation on immunogenicity. We found that 2×109 infectious units (iu) delivered intradermally conferred the most consistent and strongest responses of the different regimes tested.",

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author = "G. Dean and D. Clifford and S. Gilbert and H. McShane and Hewinson, {R. G.} and Vordermeier, {H. M.} and B. Villarreal-Ramos",

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Dean, G, Clifford, D, Gilbert, S, McShane, H, Hewinson, RG, Vordermeier, HM & Villarreal-Ramos, B 2014, 'Effect of dose and route of immunisation on the immune response induced in cattle by heterologous Bacille Calmette-Guerin priming and recombinant adenoviral vector boosting', Veterinary Immunology and Immunopathology, cyfrol. 158, rhif 3-4, tt. 208-213. https://doi.org/10.1016/j.vetimm.2014.01.010

Effect of dose and route of immunisation on the immune response induced in cattle by heterologous Bacille Calmette-Guerin priming and recombinant adenoviral vector boosting. / Dean, G.; Clifford, D.; Gilbert, S. et al.
Yn: Veterinary Immunology and Immunopathology, Cyfrol 158, Rhif 3-4, 15.04.2014, t. 208-213.

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

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AU - Dean, G.

AU - Clifford, D.

AU - Gilbert, S.

AU - McShane, H.

AU - Hewinson, R. G.

AU - Vordermeier, H. M.

AU - Villarreal-Ramos, B.

PY - 2014/4/15

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AB - BCG is used experimentally as a vaccine against tuberculosis (TB), induced by Mycobacterium bovis, in cattle (bTB). However, the efficacy of BCG is variable in humans, cattle and guinea pigs. An adenoviral vector expressing Antigen 85A (Ad5Ag85A) has enhanced protection against TB in mice when used in combination with BCG for prime-boost experiments. However, the route of immunisation affects the degree of protection seen. This work examines the immunogenicity of a new vectored vaccine (Ad5-TBF) that expresses Ag85A, Rv0287, Rv0288 and Rv0251c to explore the effects of dose of adenoviral boost and route of inoculation on immunogenicity. We found that 2×109 infectious units (iu) delivered intradermally conferred the most consistent and strongest responses of the different regimes tested.

KW - Adenovirus

KW - Mycobacteria

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Dean G, Clifford D, Gilbert S, McShane H, Hewinson RG, Vordermeier HM et al. Effect of dose and route of immunisation on the immune response induced in cattle by heterologous Bacille Calmette-Guerin priming and recombinant adenoviral vector boosting. Veterinary Immunology and Immunopathology. 2014 Ebr 15;158(3-4):208-213. Epub 2014 Ion 28. doi: 10.1016/j.vetimm.2014.01.010

Effect of dose and route of immunisation on the immune response induced in cattle by heterologous Bacille Calmette-Guerin priming and recombinant adenoviral vector boosting

Crynodeb

NDC y CU

Mynediad at Ddogfen

Cysylltiad parhaol

Ffeiliau eraill a chysylltiadau

Ôl bys

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