TY - JOUR
T1 - Effect of variation in the proportion of solid- and liquid-associated rumen bacteria in duodenal contents on the estimation of duodenal bacterial nitrogen flow
AU - Dewhurst, Richard J.
AU - Vlaeminck, B.
AU - Demeyer, D.
AU - Fievez, V.
N1 - Vlaeminck, B., Fievez, V., Demeyer, D., Dewhurst, R. J. (2007). Effect of variation in the proportion of solid- and liquid-associated rumen bacteria in duodenal contents on the estimation of duodenal bacterial nitrogen flow. Journal of Animal and Feed Sciences, 16, (1), 37-42
PY - 2007
Y1 - 2007
N2 - The aim of this research was to examine to what extent variation in the relative proportions of solid- (SAB) and liquid-associated rumen bacteria (LAB) in duodenal bacteria have an impact on the estimation of duodenal flowofbacterialN.Forthis,fourdairycowswerefeddietsvaryinginforage:concentrate ratio (80:20, 65:35, 50:50 and 35:65). SAB and LAB were separated from rumen contents four h after the morning feeding. Adenine, cytosine and odd and branched-chain fatty acids were determined both in SAB and LAB and used to estimate bacterial N flow. Bacterial N flowswerealsocalculated using a SAB:LAB ratio in duodenal bacteria, as estimated from the odd and branched-chain fatty acid pattern. Compared with calculations based on the estimated SAB:LAB ratio, estimations based on SAB or LAB only as a bacterial reference on average over- and underestimated bacterial N flowby37and55gN/d,respectively(P
AB - The aim of this research was to examine to what extent variation in the relative proportions of solid- (SAB) and liquid-associated rumen bacteria (LAB) in duodenal bacteria have an impact on the estimation of duodenal flowofbacterialN.Forthis,fourdairycowswerefeddietsvaryinginforage:concentrate ratio (80:20, 65:35, 50:50 and 35:65). SAB and LAB were separated from rumen contents four h after the morning feeding. Adenine, cytosine and odd and branched-chain fatty acids were determined both in SAB and LAB and used to estimate bacterial N flow. Bacterial N flowswerealsocalculated using a SAB:LAB ratio in duodenal bacteria, as estimated from the odd and branched-chain fatty acid pattern. Compared with calculations based on the estimated SAB:LAB ratio, estimations based on SAB or LAB only as a bacterial reference on average over- and underestimated bacterial N flowby37and55gN/d,respectively(P
M3 - Article
SN - 2719-8448
SP - 37
EP - 42
JO - Journal of Animal and Feed Sciences
JF - Journal of Animal and Feed Sciences
ER -