ELISPOT assays to enumerate bovine IFN-γ-secreting cells for the development of novel vaccines against bovine tuberculosis

Martin Vordermeier; Adam O. Whelan

doi:10.1007/978-1-61779-325-7_17

ELISPOT assays to enumerate bovine IFN-γ-secreting cells for the development of novel vaccines against bovine tuberculosis

Martin Vordermeier^*, Adam O. Whelan

^*Awdur cyfatebol y gwaith hwn

Animal Health and Veterinary Laboratories Agency

Allbwn ymchwil: Pennod mewn Llyfr/Adroddiad/Trafodion Cynhadledd › Pennod

10 Dyfyniadau (Scopus)

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Enumeration of antigen-specific cells after vaccination is one of the prime immunological parameters determined when developing vaccines. Due to their exquisite sensitivity (limits of detection can be below 1/100,000 cells), ELISPOT assays are therefore an important tool in vaccine development programs. This is particularly the case for vaccines against diseases that require protective cell-mediated immunity, such as tuberculosis. This chapter describes ELISPOT assays detecting bovine IFN-γ.

Iaith wreiddiol	Saesneg
Teitl	Handbook of ELISPOT
Is-deitl	Methods and Protocols
Golygyddion	Alexander Kalyuzhny
Tudalennau	219-227
Nifer y tudalennau	9
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1007/978-1-61779-325-7_17
Statws	Cyhoeddwyd - 01 Ion 2012

Cyfres gyhoeddiadau

Enw	Methods in Molecular Biology
Cyfrol	792
ISSN (Argraffiad)	1064-3745

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10.1007/978-1-61779-325-7_17

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Link to publication in Scopus

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abstract = "Enumeration of antigen-specific cells after vaccination is one of the prime immunological parameters determined when developing vaccines. Due to their exquisite sensitivity (limits of detection can be below 1/100,000 cells), ELISPOT assays are therefore an important tool in vaccine development programs. This is particularly the case for vaccines against diseases that require protective cell-mediated immunity, such as tuberculosis. This chapter describes ELISPOT assays detecting bovine IFN-γ.",

keywords = "Cultured ELISPOT, Cytokines, Effector and memory T-cell responses, ELISPOT, IFN-γ",

author = "Martin Vordermeier and Whelan, {Adam O.}",

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ELISPOT assays to enumerate bovine IFN-γ-secreting cells for the development of novel vaccines against bovine tuberculosis. / Vordermeier, Martin; Whelan, Adam O.
Handbook of ELISPOT: Methods and Protocols. gol. / Alexander Kalyuzhny. 2012. t. 219-227 (Methods in Molecular Biology; Cyfrol 792).

Allbwn ymchwil: Pennod mewn Llyfr/Adroddiad/Trafodion Cynhadledd › Pennod

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ELISPOT assays to enumerate bovine IFN-γ-secreting cells for the development of novel vaccines against bovine tuberculosis

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