Evaluation of a membrane filtration method for the rapid enumeration of confirmed Clostridium perfringens from water

J. Watkins; D. P. Sartory

doi:10.1111/lam.12383

Evaluation of a membrane filtration method for the rapid enumeration of confirmed Clostridium perfringens from water

J. Watkins, D. P. Sartory

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A modification of the UK reference and ISO 14189 TSCA medium for the enumeration of Clostridium perfringens from water coupled with a membrane filter transfer technique for testing for production of acid phosphatase was evaluated. The new tryptose cycloserine agar (TCA) medium, which lacks sodium metabisulphite but contains sodium pyruvate to improve recovery, allows the isolation and confirmation of Cl. perfringens within 18–24 h of sample processing. Data from a multilaboratory study analysed according to ISO 17994 showed that TCA was equivalent to TSCA for the enumeration of Cl. perfringens. The identification of acid phosphatase-negative isolates revealed a false-negative rate for the TCA method of 0·8%. The TCA membrane filter transfer procedure provides confirmed Cl. perfringens counts in half the time of the TSCA method and is simple to undertake.

Iaith wreiddiol	Saesneg
Tudalennau (o-i)	367-371
Nifer y tudalennau	5
Cyfnodolyn	Letters in Applied Microbiology
Cyfrol	60
Rhif cyhoeddi	4
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1111/lam.12383
Statws	Cyhoeddwyd - 15 Maw 2015
Cyhoeddwyd yn allanol	Ie

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10.1111/lam.12383

https://www.scopus.com/inward/record.uri?eid=2-s2.0-84924811818&doi=10.1111%2flam.12383&partnerID=40&md5=c9be59972559b6aea6ed804bf00907ae

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title = "Evaluation of a membrane filtration method for the rapid enumeration of confirmed Clostridium perfringens from water",

abstract = "A modification of the UK reference and ISO 14189 TSCA medium for the enumeration of Clostridium perfringens from water coupled with a membrane filter transfer technique for testing for production of acid phosphatase was evaluated. The new tryptose cycloserine agar (TCA) medium, which lacks sodium metabisulphite but contains sodium pyruvate to improve recovery, allows the isolation and confirmation of Cl. perfringens within 18–24 h of sample processing. Data from a multilaboratory study analysed according to ISO 17994 showed that TCA was equivalent to TSCA for the enumeration of Cl. perfringens. The identification of acid phosphatase-negative isolates revealed a false-negative rate for the TCA method of 0·8%. The TCA membrane filter transfer procedure provides confirmed Cl. perfringens counts in half the time of the TSCA method and is simple to undertake.",

keywords = "Clostridium perfringens, enumeration, indicators, rapid methods, water quality",

author = "J. Watkins and Sartory, {D. P.}",

note = "cited By 0",

year = "2015",

month = mar,

day = "15",

doi = "10.1111/lam.12383",

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AU - Watkins, J.

AU - Sartory, D. P.

N1 - cited By 0

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Y1 - 2015/3/15

N2 - A modification of the UK reference and ISO 14189 TSCA medium for the enumeration of Clostridium perfringens from water coupled with a membrane filter transfer technique for testing for production of acid phosphatase was evaluated. The new tryptose cycloserine agar (TCA) medium, which lacks sodium metabisulphite but contains sodium pyruvate to improve recovery, allows the isolation and confirmation of Cl. perfringens within 18–24 h of sample processing. Data from a multilaboratory study analysed according to ISO 17994 showed that TCA was equivalent to TSCA for the enumeration of Cl. perfringens. The identification of acid phosphatase-negative isolates revealed a false-negative rate for the TCA method of 0·8%. The TCA membrane filter transfer procedure provides confirmed Cl. perfringens counts in half the time of the TSCA method and is simple to undertake.

AB - A modification of the UK reference and ISO 14189 TSCA medium for the enumeration of Clostridium perfringens from water coupled with a membrane filter transfer technique for testing for production of acid phosphatase was evaluated. The new tryptose cycloserine agar (TCA) medium, which lacks sodium metabisulphite but contains sodium pyruvate to improve recovery, allows the isolation and confirmation of Cl. perfringens within 18–24 h of sample processing. Data from a multilaboratory study analysed according to ISO 17994 showed that TCA was equivalent to TSCA for the enumeration of Cl. perfringens. The identification of acid phosphatase-negative isolates revealed a false-negative rate for the TCA method of 0·8%. The TCA membrane filter transfer procedure provides confirmed Cl. perfringens counts in half the time of the TSCA method and is simple to undertake.

KW - Clostridium perfringens

KW - enumeration

KW - indicators

KW - rapid methods

KW - water quality

U2 - 10.1111/lam.12383

DO - 10.1111/lam.12383

M3 - Article

SN - 0266-8254

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SP - 367

EP - 371

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

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Evaluation of a membrane filtration method for the rapid enumeration of confirmed Clostridium perfringens from water

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