Exploiting plant somatic hybrids for physical mappings of ESTs

J. Wardrop; J. Fuller; Wayne Powell; G. C. Machray

doi:10.1007/s00122-003-1434-6

Exploiting plant somatic hybrids for physical mappings of ESTs

J. Wardrop
, J. Fuller
, Wayne Powell
, G. C. Machray

Athrofa'r Gwyddorau Biolegol, Amgylcheddol a Gwledig

Field Crops Research Institute

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

18 Dyfyniadau (Scopus)

Crynodeb

Methods are described for the optimisation of the generation of radiation hybrids suitable for physical mapping of a plant (barley) genome. A combination of PCR-based technologies, involving the use of whole genome, mixed primer and hemi-nested primer amplifications, can greatly extend their utility for the physical mapping of expressed sequence tags (ESTs). Using panels of hybrids and ESTs, donor DNA retention and individual marker retention frequencies for the expressed portion of the barley genome in the hybrids were estimated.

Iaith wreiddiol	Saesneg
Tudalennau (o-i)	343-348
Nifer y tudalennau	6
Cyfnodolyn	Theoretical and Applied Genetics
Cyfrol	108
Rhif cyhoeddi	2
Dyddiad ar-lein cynnar	25 Medi 2003
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1007/s00122-003-1434-6
Statws	Cyhoeddwyd - 01 Ion 2004

Mynediad at Ddogfen

10.1007/s00122-003-1434-6

http://hdl.handle.net/2160/5794

Cysylltiad parhaol

Dolen barhaus

Dyfynnu hyn

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title = "Exploiting plant somatic hybrids for physical mappings of ESTs",

abstract = "Methods are described for the optimisation of the generation of radiation hybrids suitable for physical mapping of a plant (barley) genome. A combination of PCR-based technologies, involving the use of whole genome, mixed primer and hemi-nested primer amplifications, can greatly extend their utility for the physical mapping of expressed sequence tags (ESTs). Using panels of hybrids and ESTs, donor DNA retention and individual marker retention frequencies for the expressed portion of the barley genome in the hybrids were estimated.",

author = "J. Wardrop and J. Fuller and Wayne Powell and Machray, \{G. C.\}",

note = "Wardrop, J., Fuller, J., Powell, W., Machray, G.C. (2004). Exploiting plant somatic hybrids for physical mappings of ESTs. Theoretical and Applied Genetics, 108, (2), 343-348.",

year = "2004",

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doi = "10.1007/s00122-003-1434-6",

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AU - Wardrop, J.

AU - Fuller, J.

AU - Powell, Wayne

AU - Machray, G. C.

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N2 - Methods are described for the optimisation of the generation of radiation hybrids suitable for physical mapping of a plant (barley) genome. A combination of PCR-based technologies, involving the use of whole genome, mixed primer and hemi-nested primer amplifications, can greatly extend their utility for the physical mapping of expressed sequence tags (ESTs). Using panels of hybrids and ESTs, donor DNA retention and individual marker retention frequencies for the expressed portion of the barley genome in the hybrids were estimated.

AB - Methods are described for the optimisation of the generation of radiation hybrids suitable for physical mapping of a plant (barley) genome. A combination of PCR-based technologies, involving the use of whole genome, mixed primer and hemi-nested primer amplifications, can greatly extend their utility for the physical mapping of expressed sequence tags (ESTs). Using panels of hybrids and ESTs, donor DNA retention and individual marker retention frequencies for the expressed portion of the barley genome in the hybrids were estimated.

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DO - 10.1007/s00122-003-1434-6

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JF - Theoretical and Applied Genetics

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Exploiting plant somatic hybrids for physical mappings of ESTs

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