Exploiting the Brachypodium Tool Box in cereal and grass research

Luis Alejandro Jose Mur, Joel Allainguillaume, Pilar Catalán, Robert Hasterok, Glyn Jenkins, Karolina Lesniewska, Ianto Thomas, John P. Vogel

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

115 Dyfyniadau(SciVal)

Crynodeb

It is now a decade since Brachypodium distachyon (Brachypodium) was suggested as a model species for temperate grasses and cereals. Since then transformation protocols, large expressed sequence tag (EST) databases, tools for forward and reverse genetic screens, highly refined cytogenetic probes, germplasm collections and, recently, a complete genome sequence have been generated. In this review, we will describe the current status of the Brachypodium Tool Box and how it is beginning to be applied to study a range of biological traits. Further, as genomic analysis of larger cereals and forage grasses genomes are becoming easire, we will re-evaluate Brachypodium as a model species. We suggest that there remains an urgent need to employ reverse genetic and functional genomic approaches to identify the functionality of key genetic elements, which could be employed subsequently in plant breeding programmes; and a requirement for a Pooideae reference genome to aid assembling large pooid genomes. Brachypodium is an ideal system for functional genomic studies, because of its easy growth requirements, small physical stature, and rapid life cycle, coupled with the resources offered by the Brachypodium Tool Box.
Iaith wreiddiolSaesneg
Tudalennau (o-i)334-347
Nifer y tudalennau14
CyfnodolynNew Phytologist
Cyfrol191
Rhif cyhoeddi2
Dyddiad ar-lein cynnar31 Mai 2011
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - Gorff 2011

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