@inbook{7f54fb6e061a45788ecb591c5dcabb98,
title = "Exploring the potential for translational genomics approaches in forage legumes: regions of highly conserved microsynteny between white clover and Medicago truncatula revealed by BAC sequencing",
abstract = "The model legume species Medicago truncatula is a potentially useful tool for gene discovery in white clover using translational genomics strategies. A prerequisite to the practical implementation of this approach is a good understanding of the extent of conservation of gene order between the species. Previous studies have demonstrated conservation at the macrosyntenic level, but no published information exists on the extent of conserved microsynteny between these species. In a previously published study, we reported the construction of a BAC library of white clover, the end sequencing of approximately 700 clover BACs, and the comparison of these BAC-end sequences to the M. truncatula genome. We found that 14 paired BAC-ends were shown to have the equivalent pairs of M. truncatula sequence on the same M. truncatula BAC clone or contig sequence within a span of 25?200 Kb, suggesting they represent orthologous regions in the two species. In this follow-up analysis, we have chosen five of these BACs, sequenced them to approximately six-fold coverage, and compared the resulting assembled contigs to their putatively equivalent regions of M. truncatula. Highly conserved gene content and almost complete conservation of gene order and orientation for all five sequences were found, suggesting that translational genomics approaches for gene discovery using Medicago could be successful.",
author = "Melanie Febrer and Abberton, {M. T.} and Glyn Jenkins and Dan Milbourne",
note = "Febrer, M., Abberton, M. T., Jenkins, G., Milbourne , D. (2010). Exploring the potential for translational genomics approaches in forage legumes: regions of highly conserved microsynteny between white clover and Medicago truncatula revealed by BAC sequencing, In: Sustainable use of Genetic Diversity in Forage and Turf Breeding, Huyghe, C. (Ed.) Springer Netherlands, Chapter 60, (Part 5), 415-419. This book is a collection of papers presented at the 2009 meeting of the Fodder Crops and Amenity grasses section of Eucarpia. It provides a unique source of information on the most recent results on genetic diversity and breeding in forage crops and turf species from Europe and overseas. It is organised in five sections. The first section is devoted to genetic resources which are the source of diversity for breeding but also a source of information for a better preservation of diversity through biogeography approaches. The second section focuses on changes which may occur in grassland and turf swards. These swards are plant communities which are likely to experience species and genetic changes. Population genetics provides keys to understand these changes in order to take them into account during breeding and sward management. The third section documents the genetic gains achieved during breeding while the fourth one gives a detailed presentation of the results and potential of molecular biology to better use the genetic diversity. Eventually, the fifth section investigates the potential of new structures of varieties either exploiting the heterosis or the interspecific hybridization.",
year = "2010",
month = jun,
day = "10",
doi = "10.1007/978-90-481-8706-5_60",
language = "English",
isbn = "9789048187058",
pages = "415--419",
editor = "C. Huyghe",
booktitle = "Sustainable use of Genetic Diversity in Forage and Turf Breeding",
publisher = "Springer Nature",
address = "Switzerland",
}