@article{61cfef0069a5493c99afbbe79e71a9d5,
title = "Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.)",
abstract = "An expressed sequence tag (EST) library of the key grassland species perennial ryegrass (Lolium perenne L.) has been exploited as a resource for microsatellite marker development. Out of 955 simple sequence repeat (SSR) containing ESTs, 744 were used for primer design. Primer amplification was tested in eight genotypes of L. perenne and L. multiflorum representing (grand-) parents of four mapping populations and resulted in 464 successfully amplified EST-SSRs. Three hundred and six primer pairs successfully amplified products in the mapping population VrnA derived from two of the eight genotypes included in the original screening and revealed SSR polymorphisms for 143 ESTs. Here, we report on 464 EST-derived SSR primer sequences of perennial ryegrass established in laboratory assays, providing a dedicated tool for marker assisted breeding and comparative mapping within and among forage and turf grasses.",
keywords = "comparative mapping, simple sequence repeat (SSR), expressed sequence tag (EST), ryegrass (Lolium spp.)",
author = "Bruno Studer and Torben Asp and Ursula Frei and Stephan Hentrup and Helena Meally and Aur{\'e}lie Guillard and Susanne Barth and Hilde Muylle and Isabel Roldan-Ruiz and Philippe Barre and Carole Koning-Boucoiran and Gerda Uuenk-Stunnenberg and Oene Dolstra and L. Skot and Skot, {K. P.} and Turner, {L. B.} and Humphreys, {M. O.} and Roland Kolliker and Niels Roulund and Nielsen, {Klaus K.} and Thomas L{\"u}bberstedt",
note = "Studer, B., Asp, T., Frei, U., Hentrup, S. Meally, H., Guillard, A., Barth, S., Muylle, H., Roldan-Ruiz, I., Barre, P., Koning-Boucoiran, C., Uuenk-Stunnenberg, G., Dolstra, O., Skot, L., Skot, K. P., Turner, L. B., Humphreys, M. O., Kolliker, R., Roulund, N., Nielsen, K. K., Lubberstedt, T. (2008). Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.). Molecular Breeding, 21,(4), 533-548. Sponsorship: Directorate for Food, Fisheries and Agri Business (DFFE), Denmark. IMPF: 02.01 RONO: 1340 3002 ",
year = "2008",
month = may,
day = "1",
doi = "10.1007/s11032-007-9148-0",
language = "English",
volume = "21",
pages = "533--548",
journal = "Molecular Breeding",
issn = "1572-9788",
publisher = "Springer Nature",
number = "4",
}