Expression of HMW subunit, gamma-zein and uidA genes in endosperms of transgenic bread wheat

G. Y. He, R. D'Ovidio, O. D. Anderson, R. Fido, A. S. Tatham, H. Darlington, J. Napier, P. A. Lazzeri, H. D. Jones, P. R. Shewry

Allbwn ymchwil: Pennod mewn Llyfr/Adroddiad/Trafodion CynhadleddTrafodion Cynhadledd (Nid-Cyfnodolyn fathau)

Crynodeb

Transformation allows the production of wheat plants with specific differences in their gluten structure, by the introduction of additional genes for wild type or mutant proteins. Similarly, genes from other species can also be inserted, such as those for the gamma -zein proteins which are associated with hard endosperm texture in maize. It therefore allows the relationships between protein composition and grain functionality to be established. Transgenic wheat lines were obtained by transformation with genes encoding wild type subunit 1Dx5, long and short mutant forms of this subunit and subunit Dx2.2*, using particle bombardment. The mutant HMW subunit constructs differ in the lengths of their repetitive domain while subunit 1Dx2.2* is the largest HMW glutenin subunit reported. These genes will therefore provide information on the relationship between subunit structure and gluten properties. Expression of the wild type and mutant forms of subunit 1Dx5 has been shown by SDS-PAGE of seed proteins. Small-scale Mixograph analysis and other testing of flour from these lines will be carried out to determine changes in functional properties. In a related study, transgenic wheat plants with the gamma -zein gene driven by either the wheat HMW subunit promoter or the maize gamma -zein promoter were produced. Analysis of the seed proteins of several gamma -zein PCR-positive lines, by dot blotting and antibody hybridisation and SDS-PAGE, has shown the expression of the gamma -zein gene. To study the tissue specificity, several homozygous transgenic plants were produced with the uidA gene driven by the HMW subunit promoter. GUS expression was observed in starchy endosperms of transgenic plants in the T-1, T-2 and T-3 generations, but not in leaves, roots, anthers and pollen, demonstrating endosperm-specific expression regulated by the HMW subunit promoter.

Iaith wreiddiolSaesneg
TeitlWheat in a Global Environment
Is-deitlProceedings of the 6th International Wheat Conference, 5-9 June, Budapest, Hungary
GolygyddionZ. Bedo, L. Lang
CyhoeddwrSpringer Nature
Tudalennau177-181
Nifer y tudalennau3
ISBN (Argraffiad)978-0792367222, 0792367227
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 2001
Digwyddiad6th International Wheat Conference - BUDAPEST, Hwngari
Hyd: 05 Meh 200009 Meh 2000

Cyfres gyhoeddiadau

EnwDevelopments in Plant Breeding
CyhoeddwrSpringer
Cyfrol9

Cynhadledd

Cynhadledd6th International Wheat Conference
Gwlad/TiriogaethHwngari
DinasBUDAPEST
Cyfnod05 Meh 200009 Meh 2000

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