Extraction, structural characterisation and evaluation of hydroxycinnamate esters of orchard grass (Dactylis glomerata) as substrates for polyphenol oxidase

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

45 Dyfyniadau(SciVal)

Crynodeb

Polyphenol oxidase (PPO) activity has been reported in orchard grass (Dactylis glomerata); however, to date, no endogenous substrates have been identified. In the present study, we report the isolation and structural elucidation of PPO substrates in this species. The free phenol fraction was extracted, separated by reverse-phase chromatography and six potential substrates, including two hydroxycinnamate esters, were identified by UV spectrometry, electrospray ionisation-tandem mass spectrometry (LC?ESI-MSn) and 1D and 2D NMR analyses (1H NMR, 13C NMR, DEPT, COSY, HMQC and HMBC). Furthermore, three caffeoylquinic acids (3-CQA, 4-CQA and 5-CQA) were identified by comparison of their spectral data (ESI-MS) with those of known compounds and literature data. Five of these compounds were demonstrated to be substrates for orchard grass PPO.
Iaith wreiddiolSaesneg
Tudalennau (o-i)2799-2806
Nifer y tudalennau8
CyfnodolynPhytochemistry
Cyfrol69
Rhif cyhoeddi16
Dyddiad ar-lein cynnar15 Hyd 2008
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 01 Tach 2008

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Gweld gwybodaeth am bynciau ymchwil 'Extraction, structural characterisation and evaluation of hydroxycinnamate esters of orchard grass (Dactylis glomerata) as substrates for polyphenol oxidase'. Gyda’i gilydd, maen nhw’n ffurfio ôl bys unigryw.

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