Fatty acid oxidation products (‘green odour’) released from perennial ryegrass following biotic and abiotic stress, potentially have antimicrobial properties against the rumen microbiota resulting in decreased biohydrogenation

S. A. Huws, M. B. Scott, J. K. S. Tweed, M. R. F. Lee

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

9 Dyfyniadau(SciVal)

Crynodeb

Aims
In this experiment, we investigated the effect of ‘green odour’ products typical of those released from fresh forage postabiotic and biotic stresses on the rumen microbiota and lipid metabolism.

Methods and Results
Hydroperoxyoctadecatrienoic acid (HP), a combination of salicylic and jasmonic acid (T), and a combination of both (HPT) were incubated in vitro in the presence of freeze-dried ground silage and rumen fluid, under rumen-like conditions. 16S rRNA (16S cDNA) HaeIII-based terminal restriction fragment length polymorphism-based (T-RFLP) dendrograms, canonical analysis of principal coordinates graphs, peak number and Shanon-Weiner diversity indices show that HP, T and HPT likely had antimicrobial effects on the microbiota compared to control incubations. Following 6 h of in vitro incubation, 15·3% of 18:3n-3 and 4·4% of 18:2n-6 was biohydrogenated in control incubations, compared with 1·3, 9·4 and 8·3% of 18:3n-3 for HP, T and HPT treatments, respectively, with negligible 18:2n-6 biohydrogenation seen. T-RFLP peaks lost due to application of HP, T and HPT likely belonged to as yet uncultured bacteria within numerous genera.

Conclusions
Hydroperoxyoctadecatrienoic acid, T and HPT released due to plant stress potentially have an antimicrobial effect on the rumen microbiota, which may explain the decreased biohydrogenation in vitro.

Significance and Impact of the Study
These data suggest that these volatile chemicals may be responsible for the higher summer n-3 content of bovine milk.
Iaith wreiddiolSaesneg
Tudalennau (o-i)1081-1090
CyfnodolynJournal of Applied Microbiology
Cyfrol115
Rhif cyhoeddi5
Dyddiad ar-lein cynnar21 Awst 2013
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - Tach 2013

Ôl bys

Gweld gwybodaeth am bynciau ymchwil 'Fatty acid oxidation products (‘green odour’) released from perennial ryegrass following biotic and abiotic stress, potentially have antimicrobial properties against the rumen microbiota resulting in decreased biohydrogenation'. Gyda’i gilydd, maen nhw’n ffurfio ôl bys unigryw.

Dyfynnu hyn