Flexible genes establish widespread bacteriophage pan-genomes in cryoconite hole ecosystems

Christopher M. Bellas*, Declan C. Schroeder, Arwyn Edwards, Gary Barker, Alexandre M. Anesio

*Awdur cyfatebol y gwaith hwn

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

26 Dyfyniadau(SciVal)
76 Wedi eu Llwytho i Lawr (Pure)

Crynodeb

Bacteriophage genomes rapidly evolve via mutation and horizontal gene transfer to counter evolving bacterial host defenses; such arms race dynamics should lead to divergence between phages from similar, geographically isolated ecosystems. However, near-identical phage genomes can reoccur over large geographical distances and several years apart, conversely suggesting many are stably maintained. Here, we show that phages with near-identical core genomes in distant, discrete aquatic ecosystems maintain diversity by possession of numerous flexible gene modules, where homologous genes present in the pan-genome interchange to create new phage variants. By repeatedly reconstructing the core and flexible regions of phage genomes from different metagenomes, we show a pool of homologous gene variants co-exist for each module in each location, however, the dominant variant shuffles independently in each module. These results suggest that in a natural community, recombination is the largest contributor to phage diversity, allowing a variety of host recognition receptors and genes to counter bacterial defenses to co-exist for each phage.

Iaith wreiddiolSaesneg
Rhif yr erthygl4403
Nifer y tudalennau10
CyfnodolynNature Communications
Cyfrol11
Rhif cyhoeddi1
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 02 Medi 2020

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Gweld gwybodaeth am bynciau ymchwil 'Flexible genes establish widespread bacteriophage pan-genomes in cryoconite hole ecosystems'. Gyda’i gilydd, maen nhw’n ffurfio ôl bys unigryw.

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