Genetic transformation of Dichanthium annulatum (Forssk) - an apomictic tropical forage grass

Susan Dalton; Andy J. E. Bettany; V. Bhat; M. G. Gupta; K. Bailey; Emma Timms-Taravella; Phillip Morris

doi:10.1007/s00299-003-0600-2

Genetic transformation of Dichanthium annulatum (Forssk) - an apomictic tropical forage grass

Susan Dalton, Andy J. E. Bettany, V. Bhat, M. G. Gupta, K. Bailey, Emma Timms-Taravella, Phillip Morris

Adran y Gwyddorau Bywyd

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

16 Dyfyniadau (Scopus)

Crynodeb

Eleven Dichanthium annulatum (Forssk) plants were regenerated from embryogenic callus co-transformed with two plasmids encoding either the hygromycin phosphotransferase gene ( hph) or the ß-glucuronidase(GUS) gene ( uidA). Analysis of these putative transformants showed that three plants were transformed with the hph gene, showed the presence of the hph transcript and expressed hygromycin resistance after transfer to soil. Two of these also contained the uidA gene but did not express GUS and were shown to be the same transformation event. All three of the transformants set seed. Hygromycin resistance varied from 68–100% in the progeny of the three transformants. Transgene transmission appeared to have been mainly through apomixis.

Iaith wreiddiol	Saesneg
Tudalennau (o-i)	974-980
Nifer y tudalennau	7
Cyfnodolyn	Plant Cell Reports
Cyfrol	21
Rhif cyhoeddi	10
Dyddiad ar-lein cynnar	28 Maw 2003
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1007/s00299-003-0600-2
Statws	Cyhoeddwyd - Meh 2003

Mynediad at Ddogfen

10.1007/s00299-003-0600-2

http://hdl.handle.net/2160/3440

Cysylltiad parhaol

Dolen barhaus

Dyfynnu hyn

@article{cf3f95d3f9d843b392889121d9dcfddb,

title = "Genetic transformation of Dichanthium annulatum (Forssk) - an apomictic tropical forage grass",

abstract = "Eleven Dichanthium annulatum (Forssk) plants were regenerated from embryogenic callus co-transformed with two plasmids encoding either the hygromycin phosphotransferase gene ( hph) or the {\ss}-glucuronidase(GUS) gene ( uidA). Analysis of these putative transformants showed that three plants were transformed with the hph gene, showed the presence of the hph transcript and expressed hygromycin resistance after transfer to soil. Two of these also contained the uidA gene but did not express GUS and were shown to be the same transformation event. All three of the transformants set seed. Hygromycin resistance varied from 68–100% in the progeny of the three transformants. Transgene transmission appeared to have been mainly through apomixis.",

keywords = "Dichanthium, Microprojectile bombardment, Co-transformation, Apomixis, Transgenic plants",

author = "Susan Dalton and Bettany, {Andy J. E.} and V. Bhat and Gupta, {M. G.} and K. Bailey and Emma Timms-Taravella and Phillip Morris",

note = "Dalton, S. J., Bettany, A. J. E., Bhat, V., Gupta, M. G., Bailey, K., Timms, E. J., Morris, P. (2003). Genetic transformation of Dichanthium annulatum (Forssk) - an apomictic tropical forage grass. Plant Cell Reports, 21, (10), 974-980. Sponsorship: ODA; BBSRC",

year = "2003",

month = jun,

doi = "10.1007/s00299-003-0600-2",

language = "English",

volume = "21",

pages = "974--980",

journal = "Plant Cell Reports",

issn = "0721-7714",

publisher = "Springer Nature",

number = "10",

}

TY - JOUR

T1 - Genetic transformation of Dichanthium annulatum (Forssk) - an apomictic tropical forage grass

AU - Dalton, Susan

AU - Bettany, Andy J. E.

AU - Bhat, V.

AU - Gupta, M. G.

AU - Bailey, K.

AU - Timms-Taravella, Emma

AU - Morris, Phillip

N1 - Dalton, S. J., Bettany, A. J. E., Bhat, V., Gupta, M. G., Bailey, K., Timms, E. J., Morris, P. (2003). Genetic transformation of Dichanthium annulatum (Forssk) - an apomictic tropical forage grass. Plant Cell Reports, 21, (10), 974-980. Sponsorship: ODA; BBSRC

PY - 2003/6

Y1 - 2003/6

N2 - Eleven Dichanthium annulatum (Forssk) plants were regenerated from embryogenic callus co-transformed with two plasmids encoding either the hygromycin phosphotransferase gene ( hph) or the ß-glucuronidase(GUS) gene ( uidA). Analysis of these putative transformants showed that three plants were transformed with the hph gene, showed the presence of the hph transcript and expressed hygromycin resistance after transfer to soil. Two of these also contained the uidA gene but did not express GUS and were shown to be the same transformation event. All three of the transformants set seed. Hygromycin resistance varied from 68–100% in the progeny of the three transformants. Transgene transmission appeared to have been mainly through apomixis.

AB - Eleven Dichanthium annulatum (Forssk) plants were regenerated from embryogenic callus co-transformed with two plasmids encoding either the hygromycin phosphotransferase gene ( hph) or the ß-glucuronidase(GUS) gene ( uidA). Analysis of these putative transformants showed that three plants were transformed with the hph gene, showed the presence of the hph transcript and expressed hygromycin resistance after transfer to soil. Two of these also contained the uidA gene but did not express GUS and were shown to be the same transformation event. All three of the transformants set seed. Hygromycin resistance varied from 68–100% in the progeny of the three transformants. Transgene transmission appeared to have been mainly through apomixis.

KW - Dichanthium

KW - Microprojectile bombardment

KW - Co-transformation

KW - Apomixis

KW - Transgenic plants

U2 - 10.1007/s00299-003-0600-2

DO - 10.1007/s00299-003-0600-2

M3 - Article

SN - 0721-7714

VL - 21

SP - 974

EP - 980

JO - Plant Cell Reports

JF - Plant Cell Reports

IS - 10

ER -

Genetic transformation of Dichanthium annulatum (Forssk) - an apomictic tropical forage grass

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