TY - JOUR
T1 - Genetic variability and chromosome-length polymorphisms of the witches' broom pathogen Crinipellis perniciosa from various plant hosts in South America
AU - Rincones, Johana
AU - Mazotti, Gabriel D.
AU - Griffith, Gareth W.
AU - Pomela, Aalan
AU - Figueira, Antonio
AU - Leal Jr, Gildemberg A.
AU - Queiroz, Marisa V.
AU - Pereira, Jorge F.
AU - Azevedo, Ricardo A.
AU - Pereira, Goncalo A.
AU - Meinhardt, Lyndel W.
N1 - Rincones, J., Mazotti, G. D., Griffith, G. W., Pomela, A., Figueira, A., Leal, G. A. Jr., Queiroz, M. V., Pereira, J. F., Azevedo, R. A., Pereira, G. A., Meinhardt, L. W. (2006). Genetic variability and chromosome-length polymorphisms of the witches' broom pathogen Crinipellis perniciosa from various plant hosts in South America. Mycological Research, 110, (7), 821-832.
Sponsorship: FAPESP (Fundac¸a˜o de Amparo a` Pesquisa do Estado de Sa˜o Paulo, Nos. 00/10545-4 amd 02/09280-1) CNPq (Conselho Nacional de Pesquisa e Desenvolvimento, Nos. 68.0032/01-0; 47.1609/03-0) and SEAGRI (Secretaria de Agricultura do Estado da Bahia
PY - 2006/7
Y1 - 2006/7
N2 - Crinipellis perniciosa has been classified into at least four known biotypes associated with members of unrelated plant families. In this study, genetic variability is shown for 27 C (Cacao), 4 S (Solanum), and 7 L biotype (Liana) isolates of C. perniciosa collected from different regions of Brazil and South America. The objective was to investigate the genetic variability of the pathogen in the cacao-producing region of Bahia, Brazil, and elsewhere, through microsatellite analysis, and attempt to identify possible correlations between host specificity and electrophoretic karyotypes. The PCR-banding patterns were found to vary both within and between the different biotypes, and a correlation was established between the PCR-banding patterns and the chromosomal-banding patterns of each isolate. Microsatellite and chromosomal patterns among all of the L and S biotype isolates were distinctly different from the C biotypes analysed. A higher degree of genetic and chromosomal variability was found among C biotype isolates from the Amazon in comparison with C biotype isolates from Bahia, which seems to be comprised of only two main genotypes. This finding has important implications to the current cacao-breeding programme in Brazil.
AB - Crinipellis perniciosa has been classified into at least four known biotypes associated with members of unrelated plant families. In this study, genetic variability is shown for 27 C (Cacao), 4 S (Solanum), and 7 L biotype (Liana) isolates of C. perniciosa collected from different regions of Brazil and South America. The objective was to investigate the genetic variability of the pathogen in the cacao-producing region of Bahia, Brazil, and elsewhere, through microsatellite analysis, and attempt to identify possible correlations between host specificity and electrophoretic karyotypes. The PCR-banding patterns were found to vary both within and between the different biotypes, and a correlation was established between the PCR-banding patterns and the chromosomal-banding patterns of each isolate. Microsatellite and chromosomal patterns among all of the L and S biotype isolates were distinctly different from the C biotypes analysed. A higher degree of genetic and chromosomal variability was found among C biotype isolates from the Amazon in comparison with C biotype isolates from Bahia, which seems to be comprised of only two main genotypes. This finding has important implications to the current cacao-breeding programme in Brazil.
KW - Electrophoretic karyotype
KW - Intraspecific genetic variability
KW - Microsatellite primers
KW - Pulsed-field gel electrophoresis
KW - Theobroma cacao
U2 - 10.1016/j.mycres.2006.05.002
DO - 10.1016/j.mycres.2006.05.002
M3 - Article
C2 - 16876701
SN - 0953-7562
VL - 110
SP - 821
EP - 832
JO - Mycological Research
JF - Mycological Research
IS - 7
ER -