TY - JOUR
T1 - Genetics of symbiosis in Lotus japonicus: recombinant inbred lines, comparative genetic maps and map position of 35 symbiotic loci
AU - Sandal, Niels
AU - Petersen, Thomas Rørby
AU - Murray, Jeremy
AU - Umehara, Yosuke
AU - Karas, Bogumil
AU - Yano, Koji
AU - Kumagai, Hirotaka
AU - Yoshikawa, Makoto
AU - Saito, Katsuharu
AU - Hayashi, Masaki
AU - Murakami, Yasuhiro
AU - Wang, Xinwang
AU - Hakoyama, Tsuneo
AU - Imaizumi-Anraku, Haruko
AU - Sato, Shusei
AU - Kato, Tomohiko
AU - Chen, Wenli
AU - Hossain, Md. Shakhawat
AU - Shibata, Satoshi
AU - Wang, Trevor L.
AU - Yokota, Keisuke
AU - Larsen, Knud
AU - Kanamori, Norihito
AU - Madsen, Esben
AU - Radutoiu, Simona
AU - Madsen, Lene H.
AU - Radu, Talida Gratiela
AU - Krusell, Lene
AU - Ooki, Yasuhiro
AU - Banba, Mari
AU - Betti, Marco
AU - Rispail, Nicolas
AU - Skøt, Leif
AU - Tuck, Elaine F.
AU - Perry, Jillian
AU - Yoshida, Satoko
AU - Vickers, Kate
AU - Pike, Jodie
AU - Mulder, Lonneke
AU - Charpentier, Myriam
AU - Müller, Judith
AU - Ohtomo, Ryo
AU - Kojima, Tomoko
AU - Ando, Shotaro
AU - Marquez, Antonio J.
AU - Gresshoff, Peter M.
AU - Harada, Kyuya
AU - Webb, K. Judith
AU - Hata, Shingo
AU - Suganuma, Norio
AU - Kouchi, Hiroshi
AU - Kawasaki, Shinji
AU - Tabata, Satoshi
AU - Hayashi, Makoto
AU - Parniske, Martin
AU - Szczyglowski, Krzysztof
AU - Kawaguchi, Masayoshi
AU - Stougaard, Jens
N1 - Sandal, N., Petersen, T. R., Murray, J., Umehara, Y., Karas, B., Yano, K., Kumagai, H., Yoshikawa, M., Saito, K., Hayashi, M., Murakami, Y., Wang, X., Hakoyama, T., Imaizumi-Anraku, H., Sato, S., Kato, T., Chen, W., Hossain, Md. S., Shibata, S., Wang, T. L., Yokota, K., Larsen, K., Kanamori, N., Madsen, E., Radutoiu, S., Madsen, L. H., Radu, T. G., Krusell, L., Ooki, Y., Banba, M., Betti, M., Rispail, N., Skøt, L., Tuck, E. F., Perry, J., Yoshida, S., Vickers, K., Pike, J., Mulder, L., Charpentier, M., Muller, J., Ohtomo, R., Kojima, T., Ando, S., Marquez, A. J., Gresshoff, P. M., Harada, K., Webb, K. J., Hata, S., Suganuma, N. (2006). Genetics of symbiosis in Lotus japonicus: recombinant inbred lines, comparative genetic maps and map position of 35 symbiotic loci. Molecular Plant-Microbe Interactions, 19, (1), 80-91.
Sponsorship: BBSRC / EC / University of Aberystwyth
PY - 2006/1
Y1 - 2006/1
N2 - Development of molecular tools for the analysis of the plant genetic contribution to rhizobial and mycorrhizal symbiosis has provided major advances in our understanding of plant-microbe interactions, and several key symbiotic genes have been identified and characterized. In order to increase the efficiency of genetic analysis in the model legume Lotus japonicus, we present here a selection of improved genetic tools. The two genetic linkage maps previously developed from an interspecific cross between L. japonicus Gifu and L. filicaulis, and an intraspecific cross between the two ecotypes L. japonicus Gifu and L. japonicus MG-20, were aligned through a set of anchor markers. Regions of linkage groups, where genetic resolution is obtained preferentially using one or the other parental combination, are highlighted. Additional genetic resolution and stabilized mapping populations were obtained in recombinant inbred lines derived by a single seed descent from the two populations. For faster mapping of new loci, a selection of reliable markers spread over the chromosome arms provides a common framework for more efficient identification of new alleles and new symbiotic loci among uncharacterized mutant lines. Combining resources from the Lotus community, map positions of a large collection of symbiotic loci are provided together with alleles and closely linked molecular markers. Altogether, this establishes a common genetic resource for Lotus spp. A web-based version will enable this resource to be curated and updated regularly.
AB - Development of molecular tools for the analysis of the plant genetic contribution to rhizobial and mycorrhizal symbiosis has provided major advances in our understanding of plant-microbe interactions, and several key symbiotic genes have been identified and characterized. In order to increase the efficiency of genetic analysis in the model legume Lotus japonicus, we present here a selection of improved genetic tools. The two genetic linkage maps previously developed from an interspecific cross between L. japonicus Gifu and L. filicaulis, and an intraspecific cross between the two ecotypes L. japonicus Gifu and L. japonicus MG-20, were aligned through a set of anchor markers. Regions of linkage groups, where genetic resolution is obtained preferentially using one or the other parental combination, are highlighted. Additional genetic resolution and stabilized mapping populations were obtained in recombinant inbred lines derived by a single seed descent from the two populations. For faster mapping of new loci, a selection of reliable markers spread over the chromosome arms provides a common framework for more efficient identification of new alleles and new symbiotic loci among uncharacterized mutant lines. Combining resources from the Lotus community, map positions of a large collection of symbiotic loci are provided together with alleles and closely linked molecular markers. Altogether, this establishes a common genetic resource for Lotus spp. A web-based version will enable this resource to be curated and updated regularly.
KW - symbiotic mutants
U2 - 10.1094/MPMI-19-0080
DO - 10.1094/MPMI-19-0080
M3 - Article
C2 - 16404956
SN - 0894-0282
VL - 19
SP - 80
EP - 91
JO - Molecular Plant-Microbe Interactions
JF - Molecular Plant-Microbe Interactions
IS - 1
ER -