TY - JOUR
T1 - Genome Sequence Analysis of Two Pseudomonas putida Strains to Identify a 17-Hydroxylase Putatively Involved in Sparteine Degradation
AU - Detheridge, Andrew
AU - Griffith, Gareth
AU - Hopper, David
N1 - Funding Information:
The complete genome sequences were deposited in the European Nucelotide Archive (https://www.ebi.ac.uk/ena) with the accession number for Pseudomonas putida (LUP) of ERX2741807 (PRJEB28091) and for Pseudomonas putida (SPAR) of ERX2741806 (PRJEB28090). Genome sequencing was provided by MicrobesNG (http://www.microbesng .uk), which is supported by the BBSRC (grant number BB/ L024209/1).Both cultures are deposited at the National Collection of Industrial, Food and Marine Bacteria (NCIMB Ltd., Aberdeen) with accession numbers 15126 (Psp-LUP)
Publisher Copyright:
© 2018, The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Two strains of Pseudomonas putida, Psp-LUP and Psp-SPAR, capable of growth on the quinolizidine alkaloids, lupanine and sparteine respectively, were studied here. We report the isolation of Psp-SPAR and the complete genome sequencing of both bacteria. Both were confirmed to belong to P. putida, Psp-LUP close to the type isolate of the species (NBRC14164T) and Psp-SPAR close to strains KT2440 and F1. Psp-SPAR did not grow on lupanine but did contain a gene encoding a putative quinolizidine-17-hydroxylase peptide which exhibited high similarity (76%identity) to the lupanine-17-hydroxylase characterised from Psp-LUP.
AB - Two strains of Pseudomonas putida, Psp-LUP and Psp-SPAR, capable of growth on the quinolizidine alkaloids, lupanine and sparteine respectively, were studied here. We report the isolation of Psp-SPAR and the complete genome sequencing of both bacteria. Both were confirmed to belong to P. putida, Psp-LUP close to the type isolate of the species (NBRC14164T) and Psp-SPAR close to strains KT2440 and F1. Psp-SPAR did not grow on lupanine but did contain a gene encoding a putative quinolizidine-17-hydroxylase peptide which exhibited high similarity (76%identity) to the lupanine-17-hydroxylase characterised from Psp-LUP.
KW - Alkaloids/genetics
KW - Bacterial Proteins/genetics
KW - Genome, Bacterial/genetics
KW - Oxidoreductases Acting on CH-NH Group Donors/genetics
KW - Pseudomonas putida/genetics
KW - Sequence Analysis/methods
KW - Sparteine/analogs & derivatives
KW - Steroid 17-alpha-Hydroxylase/genetics
KW - Whole Genome Sequencing/methods
UR - http://www.scopus.com/inward/record.url?scp=85054063760&partnerID=8YFLogxK
U2 - 10.1007/s00284-018-1573-2
DO - 10.1007/s00284-018-1573-2
M3 - Article
C2 - 30267141
SN - 0343-8651
VL - 75
SP - 1649
EP - 1654
JO - Current Microbiology
JF - Current Microbiology
IS - 12
ER -