TY - JOUR
T1 - Genotyping by RAD sequencing enables mapping of fatty acid composition traits in perennial ryegrass (Lolium perenne (L.))
AU - Hegarty, Matthew John
AU - Yadav, Rattan Singh
AU - Lee, Michael Richard
AU - Armstead, Ian Peter
AU - Sanderson, Ruth
AU - Scollan, Nigel David
AU - Powell, Wayne
AU - Skøt, Leif
N1 - © 2013 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.
PY - 2013/5/17
Y1 - 2013/5/17
N2 - Perennial ryegrass (Lolium perenne L.) is the most important forage crop in temperate livestock agriculture. Its nutritional quality has significant impact on the quality of meat and milk for human consumption. Evidence suggests that higher energy content in forage can assist in reducing greenhouse gas emissions from ruminants. Increasing the fatty acid content (especially α-linolenic acid, an omega-3 fatty acid) may thus contribute to better forage, but little is known about the genetic basis of variation for this trait. To this end, quantitative trait loci (QTLs) were identified associated with major fatty acid content in perennial ryegrass using a population derived from a cross between the heterozygous and outbreeding high-sugar grass variety AberMagic and an older variety, Aurora. A genetic map with 434 restriction-associated DNA (RAD) and SSR markers was generated. Significant QTLs for the content of palmitic (C16:0) on linkage groups (LGs) 2 and 7; stearic (C18:0) on LGs 3, 4 and 7; linoleic (C18:2n-6) on LGs 2 and 5; and α-linolenic acids (C18:3n-3) on LG 1 were identified. Two candidate genes (a lipase and a beta-ketoacyl CoA synthase), both associated with C16:0, and separately with C18:2n-6 and C18:0 contents, were identified. The physical positions of these genes in rice and their genetic positions in perennial ryegrass were consistent with established syntenic relationships between these two species. Validation of these associations is required, but the utility of RAD markers for rapid generation of genetic maps and QTL analysis has been demonstrated for fatty acid composition in a global forage crop.
AB - Perennial ryegrass (Lolium perenne L.) is the most important forage crop in temperate livestock agriculture. Its nutritional quality has significant impact on the quality of meat and milk for human consumption. Evidence suggests that higher energy content in forage can assist in reducing greenhouse gas emissions from ruminants. Increasing the fatty acid content (especially α-linolenic acid, an omega-3 fatty acid) may thus contribute to better forage, but little is known about the genetic basis of variation for this trait. To this end, quantitative trait loci (QTLs) were identified associated with major fatty acid content in perennial ryegrass using a population derived from a cross between the heterozygous and outbreeding high-sugar grass variety AberMagic and an older variety, Aurora. A genetic map with 434 restriction-associated DNA (RAD) and SSR markers was generated. Significant QTLs for the content of palmitic (C16:0) on linkage groups (LGs) 2 and 7; stearic (C18:0) on LGs 3, 4 and 7; linoleic (C18:2n-6) on LGs 2 and 5; and α-linolenic acids (C18:3n-3) on LG 1 were identified. Two candidate genes (a lipase and a beta-ketoacyl CoA synthase), both associated with C16:0, and separately with C18:2n-6 and C18:0 contents, were identified. The physical positions of these genes in rice and their genetic positions in perennial ryegrass were consistent with established syntenic relationships between these two species. Validation of these associations is required, but the utility of RAD markers for rapid generation of genetic maps and QTL analysis has been demonstrated for fatty acid composition in a global forage crop.
KW - Fatty acids
KW - lipids
KW - perennial ryegrass (Lolium perenne)
KW - mapping
KW - quantitative trait loci
KW - restriction-associated DNA
KW - Quantitative trait loci
KW - Perennial ryegrass (Lolium perenne)
KW - Restriction-associated DNA
KW - Lipids
KW - Mapping
KW - Lolium/genetics
KW - Humans
KW - Molecular Sequence Data
KW - Restriction Mapping
KW - Lod Score
KW - Microsatellite Repeats/genetics
KW - Statistics, Nonparametric
KW - Plant Proteins/chemistry
KW - Amino Acid Sequence
KW - Fatty Acids/metabolism
KW - Genetic Association Studies
KW - Quantitative Trait, Heritable
KW - DNA, Plant/genetics
KW - Chromosome Mapping
KW - Genetic Markers
KW - Sequence Analysis, DNA
KW - Phenotype
KW - Quantitative Trait Loci/genetics
KW - Genotyping Techniques/methods
KW - Crosses, Genetic
UR - http://www.scopus.com/inward/record.url?scp=84878011473&partnerID=8YFLogxK
U2 - 10.1111/pbi.12045
DO - 10.1111/pbi.12045
M3 - Article
C2 - 23331642
SN - 1467-7644
VL - 11
SP - 572
EP - 581
JO - Plant Biotechnology Journal
JF - Plant Biotechnology Journal
IS - 5
ER -