High throughput Agrobacterium transformation of wheat: A tool for functional genomics

H. D. Jones*, M. Wilkinson, A. Doherty, H. Wu

*Awdur cyfatebol y gwaith hwn

Allbwn ymchwil: Pennod mewn Llyfr/Adroddiad/Trafodion CynhadleddTrafodion Cynhadledd (Nid-Cyfnodolyn fathau)


At Rothamsted Research UK, we have developed a robust protocol for the Agrobacterium-mediated transformation of elite wheat and during the last year, made over one hundred independent transgenic lines in one durum and three bread wheat varieties. We are now able to provide training or generate transgenic wheat lines as a not-for-profit service (see www.bract.org). Approximately 40% of our lines contain a single, functional T-DNA insertion. This paper describes the current status of Agrobacterium transformation of wheat and some initial molecular analysis of our lines. It also describes our plan to further increase througbput using mature seed-derived callus and other approaches.

Iaith wreiddiolSaesneg
TeitlWheat Production in Stressed Environments
Is-deitlProceedings of the 7th International Wheat Conference
GolygyddionH. T. Buck, J. E. Nisi, N. Salomon
CyhoeddwrSpringer Nature
Nifer y tudalennau7
ISBN (Argraffiad)978-1-4020-5496-9, 1402054963
StatwsCyhoeddwyd - 2007
Digwyddiad7th International Wheat Conference (7 IWC) - Mar del Plata, Ariannin
Hyd: 27 Tach 200502 Rhag 2005

Cyfres gyhoeddiadau

EnwDevelopments in Plant Breeding
ISSN (Argraffiad)1381-673X


Cynhadledd7th International Wheat Conference (7 IWC)
DinasMar del Plata
Cyfnod27 Tach 200502 Rhag 2005

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