Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis

Konstantin P. Lyashchenko; Adrian Grandison; Karen Keskinen; Alina Sikar-gang; Paul Lambotte; Javan Esfandiari; Gregory C. Ireton; Aarthy Vallur; Steven G. Reed; Gareth Jones; H. Martin Vordermeier; Judy R. Stabel; Tyler C. Thacker; Mitchell V. Palmer; W. Ray Waters; Patricia P. Wilkins

doi:10.1128/CVI.00259-17

Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis

Konstantin P. Lyashchenko, Adrian Grandison, Karen Keskinen, Alina Sikar-gang, Paul Lambotte, Javan Esfandiari, Gregory C. Ireton, Aarthy Vallur, Steven G. Reed, Gareth Jones, H. Martin Vordermeier, Judy R. Stabel, Tyler C. Thacker, Mitchell V. Palmer, W. Ray Waters, Patricia P. Wilkins (Golygydd)

TB buchol, clefydau a rheolaeth

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

19 Dyfyniadau (Scopus)

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Bovine tuberculosis (TB), caused by Mycobacterium bovis, remains an important zoonotic disease posing a serious threat to livestock and wildlife. The current TB tests relying on cell-mediated and humoral immune responses in cattle have performance limitations. To identify new serodiagnostic markers of bovine TB, we screened a panel of 101 recombinant proteins, including 10 polyepitope fusions, by a multiantigen print immunoassay (MAPIA) with well-characterized serum samples serially collected from cattle with experimental or naturally acquired M. bovis infection. A novel set of 12 seroreactive antigens was established. Evaluation of selected proteins in the dual-path platform (DPP) assay showed that the highest diagnostic accuracy (∼95%) was achieved with a cocktail of five best-performing antigens, thus demonstrating the potential for development of an improved and more practical serodiagnostic test for bovine TB.

Iaith wreiddiol	Saesneg
Rhif yr erthygl	e00259-17
Nifer y tudalennau	9
Cyfnodolyn	Clinical and Vaccine Immunology
Cyfrol	24
Rhif cyhoeddi	12
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1128/CVI.00259-17
Statws	Cyhoeddwyd - 05 Rhag 2017

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Mae’r allbwn hwn yn cyfrannu at y Nod(au) Datblygu Cynaliadwy canlynol

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10.1128/CVI.00259-17

https://cvi.asm.org/content/24/12/e00259-17

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Dyfynnu hyn

Lyashchenko, K. P., Grandison, A., Keskinen, K., Sikar-gang, A., Lambotte, P., Esfandiari, J., Ireton, G. C., Vallur, A., Reed, S. G., Jones, G., Vordermeier, H. M., Stabel, J. R., Thacker, T. C., Palmer, M. V., Waters, W. R., & Wilkins, P. P. (Gol.) (2017). Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis. Clinical and Vaccine Immunology, 24(12), erthygl e00259-17. https://doi.org/10.1128/CVI.00259-17

@article{a85de33464a240b49bcf8aeeb9407dc8,

title = "Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis",

abstract = "Bovine tuberculosis (TB), caused by Mycobacterium bovis, remains an important zoonotic disease posing a serious threat to livestock and wildlife. The current TB tests relying on cell-mediated and humoral immune responses in cattle have performance limitations. To identify new serodiagnostic markers of bovine TB, we screened a panel of 101 recombinant proteins, including 10 polyepitope fusions, by a multiantigen print immunoassay (MAPIA) with well-characterized serum samples serially collected from cattle with experimental or naturally acquired M. bovis infection. A novel set of 12 seroreactive antigens was established. Evaluation of selected proteins in the dual-path platform (DPP) assay showed that the highest diagnostic accuracy (∼95%) was achieved with a cocktail of five best-performing antigens, thus demonstrating the potential for development of an improved and more practical serodiagnostic test for bovine TB.",

author = "Lyashchenko, {Konstantin P.} and Adrian Grandison and Karen Keskinen and Alina Sikar-gang and Paul Lambotte and Javan Esfandiari and Ireton, {Gregory C.} and Aarthy Vallur and Reed, {Steven G.} and Gareth Jones and Vordermeier, {H. Martin} and Stabel, {Judy R.} and Thacker, {Tyler C.} and Palmer, {Mitchell V.} and Waters, {W. Ray} and Wilkins, {Patricia P.}",

year = "2017",

month = dec,

day = "5",

doi = "10.1128/CVI.00259-17",

language = "English",

volume = "24",

journal = "Clinical and Vaccine Immunology",

issn = "1556-6811",

publisher = "American Society for Microbiology",

number = "12",

}

Lyashchenko, KP, Grandison, A, Keskinen, K, Sikar-gang, A, Lambotte, P, Esfandiari, J, Ireton, GC, Vallur, A, Reed, SG, Jones, G, Vordermeier, HM, Stabel, JR, Thacker, TC, Palmer, MV, Waters, WR & Wilkins, PP (gol.) 2017, 'Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis', Clinical and Vaccine Immunology, cyfrol. 24, rhif 12, e00259-17. https://doi.org/10.1128/CVI.00259-17

TY - JOUR

T1 - Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis

AU - Lyashchenko, Konstantin P.

AU - Grandison, Adrian

AU - Keskinen, Karen

AU - Sikar-gang, Alina

AU - Lambotte, Paul

AU - Esfandiari, Javan

AU - Ireton, Gregory C.

AU - Vallur, Aarthy

AU - Reed, Steven G.

AU - Jones, Gareth

AU - Vordermeier, H. Martin

AU - Stabel, Judy R.

AU - Thacker, Tyler C.

AU - Palmer, Mitchell V.

AU - Waters, W. Ray

A2 - Wilkins, Patricia P.

PY - 2017/12/5

Y1 - 2017/12/5

N2 - Bovine tuberculosis (TB), caused by Mycobacterium bovis, remains an important zoonotic disease posing a serious threat to livestock and wildlife. The current TB tests relying on cell-mediated and humoral immune responses in cattle have performance limitations. To identify new serodiagnostic markers of bovine TB, we screened a panel of 101 recombinant proteins, including 10 polyepitope fusions, by a multiantigen print immunoassay (MAPIA) with well-characterized serum samples serially collected from cattle with experimental or naturally acquired M. bovis infection. A novel set of 12 seroreactive antigens was established. Evaluation of selected proteins in the dual-path platform (DPP) assay showed that the highest diagnostic accuracy (∼95%) was achieved with a cocktail of five best-performing antigens, thus demonstrating the potential for development of an improved and more practical serodiagnostic test for bovine TB.

AB - Bovine tuberculosis (TB), caused by Mycobacterium bovis, remains an important zoonotic disease posing a serious threat to livestock and wildlife. The current TB tests relying on cell-mediated and humoral immune responses in cattle have performance limitations. To identify new serodiagnostic markers of bovine TB, we screened a panel of 101 recombinant proteins, including 10 polyepitope fusions, by a multiantigen print immunoassay (MAPIA) with well-characterized serum samples serially collected from cattle with experimental or naturally acquired M. bovis infection. A novel set of 12 seroreactive antigens was established. Evaluation of selected proteins in the dual-path platform (DPP) assay showed that the highest diagnostic accuracy (∼95%) was achieved with a cocktail of five best-performing antigens, thus demonstrating the potential for development of an improved and more practical serodiagnostic test for bovine TB.

U2 - 10.1128/CVI.00259-17

DO - 10.1128/CVI.00259-17

M3 - Article

C2 - 28978510

SN - 1556-6811

VL - 24

JO - Clinical and Vaccine Immunology

JF - Clinical and Vaccine Immunology

IS - 12

M1 - e00259-17

ER -

Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis

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