Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis

Konstantin P. Lyashchenko, Adrian Grandison, Karen Keskinen, Alina Sikar-gang, Paul Lambotte, Javan Esfandiari, Gregory C. Ireton, Aarthy Vallur, Steven G. Reed, Gareth Jones, H. Martin Vordermeier, Judy R. Stabel, Tyler C. Thacker, Mitchell V. Palmer, W. Ray Waters, Patricia P. Wilkins (Golygydd)

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

17 Dyfyniadau (Scopus)

Crynodeb

Bovine tuberculosis (TB), caused by Mycobacterium bovis, remains an important zoonotic disease posing a serious threat to livestock and wildlife. The current TB tests relying on cell-mediated and humoral immune responses in cattle have performance limitations. To identify new serodiagnostic markers of bovine TB, we screened a panel of 101 recombinant proteins, including 10 polyepitope fusions, by a multiantigen print immunoassay (MAPIA) with well-characterized serum samples serially collected from cattle with experimental or naturally acquired M. bovis infection. A novel set of 12 seroreactive antigens was established. Evaluation of selected proteins in the dual-path platform (DPP) assay showed that the highest diagnostic accuracy (∼95%) was achieved with a cocktail of five best-performing antigens, thus demonstrating the potential for development of an improved and more practical serodiagnostic test for bovine TB.
Iaith wreiddiolSaesneg
Rhif yr erthygle00259-17
Nifer y tudalennau9
CyfnodolynClinical and Vaccine Immunology
Cyfrol24
Rhif cyhoeddi12
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 05 Rhag 2017

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